Wide dynamic range high-speed three-dimensional quantitative OCT angiography with a hybrid-beam scan.

We demonstrate a novel hybrid-beam scanning-based quantitative optical coherence tomography angiography (OCTA) that provides high-speed wide dynamic range blood flow speed imaging. The hybrid-beam scanning scheme enables multiple OCTA image acquisitions with a wide range of multiple time intervals simultaneously providing wide dynamic range blood flow speed imaging independent of the blood vessel orientation, which was quantified over a speed range of 0.6∼104  mm/s through the blood flow phantom experiments. A fully automated high-speed hybrid-beam scanning-based quantitative OCTA system demonstrates visualization of blood flow speeds in various vessels from the main arteries to capillaries in a 4  mm×4  mm area (1024 A-lines × 512 B-scans) in vivo in 20 s, showing its potential as a useful imaging tool for various biomedical applications.