| Literature DB >> 29761383 |
Aoife Kelly1,2,3, Aleksander M Grabiec4, Mark A Travis5,6,7.
Abstract
The study of human macrophages is often hampered by access to tissue and inability of this cell type to survive in vitro following isolation. The culture of human monocyte-derived macrophages (MDMs) represents a tool to study macrophages, with monocytes known to give rise to tissue macrophages influenced by certain environmental cues. Here we describe a method of culturing monocyte-derived macrophages from CD14+ blood monocytes and polarization toward different macrophage phenotypes.Entities:
Keywords: Human; Macrophage; Monocyte; Phagocytes; Polarization
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Year: 2018 PMID： 29761383 DOI： 10.1007/978-1-4939-7837-3_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745