Carola Analía Torres1, Cristina Marisel Pérez Zamora2, María Beatriz Nuñez3, Ana María Gonzalez4. 1. Laboratory of Microbiology and Pharmaceutical Technology, Department of Basic and Applied Sciences, National University of Chaco Austral, Presidencia Roque Sáenz Peña 3700, Chaco, Argentina; National Council for Scientific and Technical Research (CONICET), Godoy Cruz 2290, Buenos Aires C1425FQB, Argentina. Electronic address: carito@uncaus.edu.ar. 2. Laboratory of Microbiology and Pharmaceutical Technology, Department of Basic and Applied Sciences, National University of Chaco Austral, Presidencia Roque Sáenz Peña 3700, Chaco, Argentina; National Council for Scientific and Technical Research (CONICET), Godoy Cruz 2290, Buenos Aires C1425FQB, Argentina. 3. Laboratory of Microbiology and Pharmaceutical Technology, Department of Basic and Applied Sciences, National University of Chaco Austral, Presidencia Roque Sáenz Peña 3700, Chaco, Argentina. 4. National Council for Scientific and Technical Research (CONICET), Godoy Cruz 2290, Buenos Aires C1425FQB, Argentina; Institute of Botany of the Northeast (IBONE-CONICET), Sargento Juan Bautista Cabral 2131, Corrientes, Argentina.
Abstract
OBJECTIVES: This study aimed to evaluate the in vitro antioxidant capacity, to determine the anti-inflammatory effect due to lipoxygenase inhibition and to test the antimicrobial activity of ethanolic extracts from leaves of seven climbing species belonging to the Bignoniaceae family. These species are Adenocalymma marginatum (Cham.) DC., Amphilophium vauthieri DC., Cuspidaria convoluta (Vell.) A. H. Gentry, Dolichandra dentata (K. Schum.) L. G. Lohmann, Fridericia caudigera (S. Moore) L. G. Lohmann, Fridericia chica (Bonpl.) L. G. Lohmann and Tanaecium selloi (Spreng.) L. G. Lohmann. METHODS: The antioxidant activity was evaluated using three methods, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power. Lipoxygenase-inhibiting activity was assayed spectrophotometrically; the result was expressed as percent inhibition. The antimicrobial activity was assessed using the agar disk diffusion method. Minimal inhibitory concentration (MIC) and minimal bactericidal/fungicidal concentration were also determined for each extract against 12 pathogenic bacterial strains of Staphylococcus aureus and seven fungal strains of the Candida genus. The identification of the major compounds present in the most promising extract was established by high-performance liquid chromatography-tandem mass spectrometry. RESULTS: C. convoluta, F. caudigera, and F. chica exhibited the best antioxidant activity by scavenging DPPH and ABTS+ radicals and reducing Fe3+ ion. These extracts showed a notable inhibition of lipoxygenase. F. caudigera was found to have the lower MIC value against S. aureus strains and six Candida species. The extracts of F. caudigera and C. convoluta were active even against methicillin-resistant S. aureus. C. convoluta had higher total phenol content, better antioxidant activity and superior anti-inflammatory and antimicrobial activity. The main phenolic compounds found in this extract were coumaric and hydroxybenzoic acid derivatives and glycosylated and nonglycosylated flavones. CONCLUSION: Most of the extracts exhibited antioxidant activity as well as in vitro inhibition of lipoxygenase. The excellent antimicrobial activity of T. selloi and F. chica supports their use in traditional medicine as antiseptic agents. The extracts of F. caudigera and C. convoluta, both with notable biological activities in this study, could be used as herbal remedies for skin care. In addition, this study provides, for the first time, information about phenolic compounds present in C. convoluta.
OBJECTIVES: This study aimed to evaluate the in vitro antioxidant capacity, to determine the anti-inflammatory effect due to lipoxygenase inhibition and to test the antimicrobial activity of ethanolic extracts from leaves of seven climbing species belonging to the Bignoniaceae family. These species are Adenocalymma marginatum (Cham.) DC., Amphilophium vauthieri DC., Cuspidaria convoluta (Vell.) A. H. Gentry, Dolichandra dentata (K. Schum.) L. G. Lohmann, Fridericia caudigera (S. Moore) L. G. Lohmann, Fridericia chica (Bonpl.) L. G. Lohmann and Tanaecium selloi (Spreng.) L. G. Lohmann. METHODS: The antioxidant activity was evaluated using three methods, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power. Lipoxygenase-inhibiting activity was assayed spectrophotometrically; the result was expressed as percent inhibition. The antimicrobial activity was assessed using the agar disk diffusion method. Minimal inhibitory concentration (MIC) and minimal bactericidal/fungicidal concentration were also determined for each extract against 12 pathogenic bacterial strains of Staphylococcus aureus and seven fungal strains of the Candida genus. The identification of the major compounds present in the most promising extract was established by high-performance liquid chromatography-tandem mass spectrometry. RESULTS:C. convoluta, F. caudigera, and F. chica exhibited the best antioxidant activity by scavenging DPPH and ABTS+ radicals and reducing Fe3+ ion. These extracts showed a notable inhibition of lipoxygenase. F. caudigera was found to have the lower MIC value against S. aureus strains and six Candida species. The extracts of F. caudigera and C. convoluta were active even against methicillin-resistant S. aureus. C. convoluta had higher total phenol content, better antioxidant activity and superior anti-inflammatory and antimicrobial activity. The main phenolic compounds found in this extract were coumaric and hydroxybenzoic acid derivatives and glycosylated and nonglycosylated flavones. CONCLUSION: Most of the extracts exhibited antioxidant activity as well as in vitro inhibition of lipoxygenase. The excellent antimicrobial activity of T. selloi and F. chica supports their use in traditional medicine as antiseptic agents. The extracts of F. caudigera and C. convoluta, both with notable biological activities in this study, could be used as herbal remedies for skin care. In addition, this study provides, for the first time, information about phenolic compounds present in C. convoluta.
Authors: Muhammad Azam Ali; Muhammad Azmat Ullah Khan; Abdul Qayyum Rao; Adnan Iqbal; Salah Ud Din; Ahmad Ali Shahid Journal: PLoS One Date: 2021-05-04 Impact factor: 3.240
Authors: Carolina A de Lima; Mayra C Z Cubero; Yollanda E M Franco; Carla D P Rodrigues; Jessyane R do Nascimento; Débora B Vendramini-Costa; Juliana M Sciani; Cláudia Q da Rocha; Giovanna B Longato Journal: Biomed Res Int Date: 2022-02-11 Impact factor: 3.411