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Literature DB >> 2975752

Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter.

J J Janssen¹, W J van de Ven, W A van Groningen-Luyben, J Roosien, J M Vlak, W J de Grip.

Abstract

In vitro expression of cDNA encoding bovine opsin is accomplished using the baculovirus expression vector system. Full-length opsin was synthesized which was recognized by poly- and monoclonal antisera raised against bovine rhodopsin. Upon infection with a recombinant virus, 1 x 10(6) insect cells produced up to 3 micrograms opsin. Incubation of the in vitro synthesized opsin with 11-cis retinal produced a hydroxylamine-stable, photosensitive pigment.

Entities: Chemical Disease Gene Species

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Year: 1988 PMID： 2975752 DOI： 10.1007/bf00539052

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

26 in total

1. Analysis of baculovirus genomes with restriction endonucleases.

Authors: G E Smith; M D Summers
Journal: Virology Date: 1978-09 Impact factor: 3.616

2. Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin.

Authors: R A Dixon; B K Kobilka; D J Strader; J L Benovic; H G Dohlman; T Frielle; M A Bolanowski; C D Bennett; E Rands; R E Diehl; R A Mumford; E E Slater; I S Sigal; M G Caron; R J Lefkowitz; C D Strader
Journal: Nature Date: 1986 May 1-7 Impact factor: 49.962

3. cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor.

Authors: B K Kobilka; R A Dixon; T Frielle; H G Dohlman; M A Bolanowski; I S Sigal; T L Yang-Feng; U Francke; M G Caron; R J Lefkowitz
Journal: Proc Natl Acad Sci U S A Date: 1987-01 Impact factor: 11.205

1. Point mutations in bovine opsin can be classified in four groups with respect to their effect on the biosynthetic pathway of opsin.

Authors: G L DeCaluwé; W J DeGrip
Journal: Biochem J Date: 1996-12-15 Impact factor: 3.857

Review 2. Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.

Authors: Willem J de Grip; Srividya Ganapathy
Journal: Front Chem Date: 2022-06-22 Impact factor: 5.545

2 in total

Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter.

1. Analysis of baculovirus genomes with restriction endonucleases.

2. Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin.

3. cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor.

4. Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor.

5. Isolation, sequence analysis, and intron-exon arrangement of the gene encoding bovine rhodopsin.

6. Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes.

7. Production of human alpha-interferon in silkworm using a baculovirus vector.

8. Established insect cell line from the cabbage looper, Trichoplusia ni.

9. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae).

10. Expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector.

1. Point mutations in bovine opsin can be classified in four groups with respect to their effect on the biosynthetic pathway of opsin.

Review 2. Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.