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Literature DB >> 29756279

A Designed Enzyme Promotes Selective Post-translational Acylation.

Pallavi M Gosavi¹, Megha Jayachandran¹, Joel J L Rempillo¹, Oleksii Zozulia¹, Olga V Makhlynets¹, Ivan V Korendovych¹.

Abstract

A computationally designed, allosterically regulated catalyst (CaM M144H) produced by substituting a single residue in calmodulin, a non-enzymatic protein, is capable of efficient and site selective post-translational acylation of lysines in peptides with highly diverse sequences. Calmodulin's binding partners are involved in regulating a large number of cellular processes; this new chemical-biology tool will help to identify them and provide structural insight into their interactions with calmodulin.

Entities: Chemical Gene Mutation Species

Keywords: acyl transfer; allostery; calmodulin; post-translational modifications; protein design

Mesh：

Substances：
Calmodulin
Peptides
Lysine

Year: 2018 PMID： 29756279 PMCID： PMC6394838 DOI： 10.1002/cbic.201800196

Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164

49 in total

Review 1. Acetylation: a regulatory modification to rival phosphorylation?

Authors: T Kouzarides
Journal: EMBO J Date: 2000-03-15 Impact factor: 11.598

Review 2. Protein posttranslational modifications: the chemistry of proteome diversifications.

Authors: Christopher T Walsh; Sylvie Garneau-Tsodikova; Gregory J Gatto
Journal: Angew Chem Int Ed Engl Date: 2005-12-01 Impact factor: 15.336

3. DNA damage activates p53 through a phosphorylation-acetylation cascade.

Authors: K Sakaguchi; J E Herrera; S Saito; T Miki; M Bustin; A Vassilev; C W Anderson; E Appella
Journal: Genes Dev Date: 1998-09-15 Impact factor: 11.361

4. The interaction of calmodulin with amphiphilic peptides.

Authors: J A Cox; M Comte; J E Fitton; W F DeGrado
Journal: J Biol Chem Date: 1985-02-25 Impact factor: 5.157

Review 5. How calmodulin binds its targets: sequence independent recognition of amphiphilic alpha-helices.

Authors: K T O'Neil; W F DeGrado
Journal: Trends Biochem Sci Date: 1990-02 Impact factor: 13.807

Review 6. Lysine acetylation and the bromodomain: a new partnership for signaling.

Authors: Xiang-Jiao Yang
Journal: Bioessays Date: 2004-10 Impact factor: 4.345

7. Interaction of calmodulin with muscle phosphofructokinase. Changes of the aggregation state, conformation and catalytic activity of the enzyme.

Authors: G W Mayr
Journal: Eur J Biochem Date: 1984-09-17

A Designed Enzyme Promotes Selective Post-translational Acylation.

Review 1. Acetylation: a regulatory modification to rival phosphorylation?

Review 2. Protein posttranslational modifications: the chemistry of proteome diversifications.

3. DNA damage activates p53 through a phosphorylation-acetylation cascade.

4. The interaction of calmodulin with amphiphilic peptides.

Review 5. How calmodulin binds its targets: sequence independent recognition of amphiphilic alpha-helices.

Review 6. Lysine acetylation and the bromodomain: a new partnership for signaling.

7. Interaction of calmodulin with muscle phosphofructokinase. Changes of the aggregation state, conformation and catalytic activity of the enzyme.

8. Synthesis of proteins by native chemical ligation.

Review 9. Histone modifications: signalling receptors and potential elements of a heritable epigenetic code.

10. Acetylation regulates cyclophilin A catalysis, immunosuppression and HIV isomerization.

1. Minimalist de novo Design of Protein Catalysts.