Juan Yang1,2, Yanni Chen3, Xiaofan Xiong1, Xiaobo Zhou2, Lin Han1, Lei Ni1, Wenjing Wang4, Xiaofei Wang1, Lingyu Zhao1, Dongdong Shao3, Chen Huang1. 1. Department of Cell Biology and Genetics, School of Basic Medical Sciences/Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, China. 2. Center of Computational Systems Medicine, School of Biomedical Informatics, University of Texas Health Science Center at Houston, Houston, 77030, USA. 3. Department of Pediatrics, Xi'an Children's Hospital, Xi'an, 710003, Shaanxi, China. 4. Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Abstract
PURPOSE: Autism spectrum disorder (ASD) is a neurological and developmental disorder that begins early in childhood and lasts throughout one's life. Early diagnosis is essential for ASD since early treatment can enable children with ASD to make significant gains in language and social skills, but remains challenging since there are currently no specific biomarkers of ASD. This study is aimed to identify serum biomarkers for ASD. EXPERIMENTAL DESIGN: Serum of Han Chinese children with ASD (n = 68) and age-matched healthy controls (n = 80) is analyzed using magnetic bead-based separation combined with mass spectrum. RESULTS: Eight potential ASD serum biomarker peaks (m/z: 3886.69, 7775.12, 2381.71, 6638.63, 3319.17, 894.34, 4968.59, and 5910.53) with higher expression in ASD group are further identified as peptide regions of plasma serine protease inhibitor precursor (SERPINA5), platelet factor 4 (PF4), fatty acid binding protein 1(FABP1), apolipoprotein C-I precursor (APOC1), alpha-fetoprotein precursor (AFP), carboxypeptidase B2 (CPB2), trace amine-associated receptor 6 (TAAR6), and isoform1 of fibrinogen alpha chain precursor (FGA). The expression of identified proteins is validated by enzyme-linked immunosorbent assay (ELISA). CONCLUSIONS AND MEDICAL RELEVANCE: These findings reveal the exceptional disease etiology of ASD from a serum proteomic perspective, and the identified proteins might be potential biomarkers for ASD diagnosis.
PURPOSE:Autism spectrum disorder (ASD) is a neurological and developmental disorder that begins early in childhood and lasts throughout one's life. Early diagnosis is essential for ASD since early treatment can enable children with ASD to make significant gains in language and social skills, but remains challenging since there are currently no specific biomarkers of ASD. This study is aimed to identify serum biomarkers for ASD. EXPERIMENTAL DESIGN: Serum of Han Chinese children with ASD (n = 68) and age-matched healthy controls (n = 80) is analyzed using magnetic bead-based separation combined with mass spectrum. RESULTS: Eight potential ASD serum biomarker peaks (m/z: 3886.69, 7775.12, 2381.71, 6638.63, 3319.17, 894.34, 4968.59, and 5910.53) with higher expression in ASD group are further identified as peptide regions of plasma serine protease inhibitor precursor (SERPINA5), platelet factor 4 (PF4), fatty acid binding protein 1(FABP1), apolipoprotein C-I precursor (APOC1), alpha-fetoprotein precursor (AFP), carboxypeptidase B2 (CPB2), trace amine-associated receptor 6 (TAAR6), and isoform1 of fibrinogen alpha chain precursor (FGA). The expression of identified proteins is validated by enzyme-linked immunosorbent assay (ELISA). CONCLUSIONS AND MEDICAL RELEVANCE: These findings reveal the exceptional disease etiology of ASD from a serum proteomic perspective, and the identified proteins might be potential biomarkers for ASD diagnosis.
Authors: Maria Jesus Herrero; Li Wang; David Hernandez-Pineda; Payal Banerjee; Heidi Y Matos; Meredith Goodrich; Aswini Panigrahi; Nathan Anthony Smith; Joshua G Corbin Journal: Front Behav Neurosci Date: 2021-08-05 Impact factor: 3.558
Authors: Marta Gallego-Paüls; Carles Hernández-Ferrer; Mariona Bustamante; Xavier Basagaña; Jose Barrera-Gómez; Chung-Ho E Lau; Alexandros P Siskos; Marta Vives-Usano; Carlos Ruiz-Arenas; John Wright; Remy Slama; Barbara Heude; Maribel Casas; Regina Grazuleviciene; Leda Chatzi; Eva Borràs; Eduard Sabidó; Ángel Carracedo; Xavier Estivill; Jose Urquiza; Muireann Coen; Hector C Keun; Juan R González; Martine Vrijheid; Léa Maitre Journal: BMC Med Date: 2021-07-22 Impact factor: 8.775
Authors: Xusu Wang; Guangquan Liu; Na Sheng; Mi Zhang; Xinxing Pan; Siyu Liu; Ke Huang; Yu Cong; Qing Xu; Xuemei Jia; Juan Xu Journal: Ann Transl Med Date: 2020-08