| Literature DB >> 29751195 |
G Girault1, P Wattiau2, M Saqib3, B Martin1, F Vorimore1, H Singha4, M Engelsma5, H J Roest5, S Spicic6, R Grunow7, N Vicari8, S C J De Keersmaecker9, N H C Roosens9, M Fabbi8, B N Tripathi4, S Zientara1, N Madani1, K Laroucau10.
Abstract
Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub-branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei.Entities:
Keywords: Burkholderia mallei; Genotyping; HRM
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Year: 2018 PMID： 29751195 DOI： 10.1016/j.meegid.2018.05.004
Source DB: PubMed Journal: Infect Genet Evol ISSN： 1567-1348 Impact factor: 3.342