Feng Liao1, Li Liu2, En Luo3, Jian Hu4. 1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China. 2. State Key Laboratory of Oral Disease and National Clinical Research Center for Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China. 3. State Key Laboratory of Oral Disease and National Clinical Research Center for Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address: Luoen521125@sina.com. 4. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address: 00008460@whu.edu.cn.
Abstract
PURPOSE: This study evaluated the role of anti-tumor immune response of curcumin on tongue squamous cell carcinama (TSCC). EXPERIMENTAL DESIGN: Cell lines (Cal 27, FaDu) and animal model (4NQO mice model) were uesd in this study. The MTT assay was used to detecte cell proliferation. The Western blotting, immunohistochemistry and immunofluorescence were used to examine the protein expression. The flow cytometry was performed to determine the number of Treg and MDSC. RESULTS: The expression of PD-L1 and p-STAT3Y705 were does-dependently inhibited in Fadu and Cal 27 cell line. The results of in vivo demonstrated that curcumin significantly attenuated tumor growth in 4NQO mice model. The expression of PD-L1 and p-STAT3Y705 were similarly decreased in vivo. Moreover, the anti-tumor immune response was remarkably improved after curcumin treatment through increasing CD8 positive T cells and decreasing Tregs and MDSCs. CONCLUSIONS: Curcumin treatment resulted in inhibition of PD-L1 and p-STAT3Y705 expression both in vitro and in vivo. Moreover, the immunosuppressive tumor microenvironment was changed after curcumin treatment. These data suggested that curcumin could effectively promote anti-tumor immune response in TSCC.
PURPOSE: This study evaluated the role of anti-tumor immune response of curcumin on tongue squamous cell carcinama (TSCC). EXPERIMENTAL DESIGN: Cell lines (Cal 27, FaDu) and animal model (4NQO mice model) were uesd in this study. The MTT assay was used to detecte cell proliferation. The Western blotting, immunohistochemistry and immunofluorescence were used to examine the protein expression. The flow cytometry was performed to determine the number of Treg and MDSC. RESULTS: The expression of PD-L1 and p-STAT3Y705 were does-dependently inhibited in Fadu and Cal 27 cell line. The results of in vivo demonstrated that curcumin significantly attenuated tumor growth in 4NQO mice model. The expression of PD-L1 and p-STAT3Y705 were similarly decreased in vivo. Moreover, the anti-tumor immune response was remarkably improved after curcumin treatment through increasing CD8 positive T cells and decreasing Tregs and MDSCs. CONCLUSIONS:Curcumin treatment resulted in inhibition of PD-L1 and p-STAT3Y705 expression both in vitro and in vivo. Moreover, the immunosuppressive tumor microenvironment was changed after curcumin treatment. These data suggested that curcumin could effectively promote anti-tumor immune response in TSCC.
Authors: Jochen Rutz; Andrea Janicova; Katja Woidacki; Felix K-H Chun; Roman A Blaheta; Borna Relja Journal: Int J Mol Sci Date: 2020-05-26 Impact factor: 5.923