Catarina Garcia1,2, Catarina Oliveira Silva2, Carlos M Monteiro3, Marisa Nicolai1, Ana Viana4, Joana M Andrade1,2, Isabel Barasoain5, Tijana Stankovic6, José Quintana7, Inmaculada Hernández7, Ignacio González7, Francisco Estévez7, Ana M Díaz-Lanza2, Catarina P Reis3, Carlos Am Afonso3, Milica Pesic6, Patrícia Rijo1,3. 1. Research Center for Biosciences & Health Technologies (CBIOS), Universidade Lusófona de Humanidades e Tecnologias, 1749-024 Lisbon, Portugal. 2. Department of Biomedical Sciences, Faculty of Pharmacy, University of Alcalá, Ctra. A2, Km 33.600 - Campus Universitario, 28871 Alcalá de Henares, Spain. 3. Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, 1649-003 Lisbon, Portugal. 4. CQB, Faculdade de Ciências, University of Lisbon, Campo Grande 1749-016, Lisbon, Portugal. 5. Centro de Investigaciones Biológicas Consejo Superior de Investigaciones Científicas Ramiro de Maeztu, 9, 28040 Madrid, Spain. 6. Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despota Stefana 142, 11060 Belgrade, Serbia. 7. Department of Biochemistry & Molecular Biology, Instituto Universitario de Investigaciones Biomédicas y Sanitarias, University of Las Palmas de Gran Canaria, Paseo Blas Cabrera Felipe s/n, 35016 Las Palmas de Gran Canaria, Spain.
Abstract
AIM: 6,7-dehydroroyleanone (DHR) is a cytotoxic abietane present in the essential oil of Plectranthus madagascariensis. METHODS/ RESULTS: Different extraction parameters were tested, and its extraction optimization was accomplished with a Clevenger apparatus-based hydrodistillation. After isolation, its effect on microtubules, P-glycoprotein and caspases was assessed on several cell lines and the compound was coupled with hybrid nanoparticles. The results show that DHR does not interfere with microtubule formation, but evades the resistance mechanisms of P-glycoprotein. Strong activation of caspases-3 and -9 indicates that DHR is able to induce apoptosis by triggering the intrinsic cell death pathway. Moreover, the assembly of DHR with hybrid nanoparticles was able to potentiate the effect of DHR in cancer cells. CONCLUSION: DHR seems to be a promising starting material with anticancer properties to further be explored.
AIM: 6,7-dehydroroyleanone (DHR) is a cytotoxic abietane present in the essential oil of Plectranthus madagascariensis. METHODS/ RESULTS: Different extraction parameters were tested, and its extraction optimization was accomplished with a Clevenger apparatus-based hydrodistillation. After isolation, its effect on microtubules, P-glycoprotein and caspases was assessed on several cell lines and the compound was coupled with hybrid nanoparticles. The results show that DHR does not interfere with microtubule formation, but evades the resistance mechanisms of P-glycoprotein. Strong activation of caspases-3 and -9 indicates that DHR is able to induce apoptosis by triggering the intrinsic cell death pathway. Moreover, the assembly of DHR with hybrid nanoparticles was able to potentiate the effect of DHR in cancer cells. CONCLUSION:DHR seems to be a promising starting material with anticancer properties to further be explored.
Authors: Epole N Ntungwe; Sofija Jovanović Stojanov; Noélia M Duarte; Nuno R Candeias; Ana M Díaz-Lanza; Máté Vágvölgyi; Attila Hunyadi; Milica Pešić; Patrícia Rijo Journal: ACS Med Chem Lett Date: 2022-03-11 Impact factor: 4.632
Authors: Vera M S Isca; Milan Sencanski; Nenad Filipovic; Daniel J V A Dos Santos; Ana Čipak Gašparović; Lucília Saraíva; Carlos A M Afonso; Patrícia Rijo; Alfonso T García-Sosa Journal: Int J Mol Sci Date: 2020-05-23 Impact factor: 5.923
Authors: Catarina Garcia; Vera M S Isca; Filipe Pereira; Carlos M Monteiro; Epole Ntungwe; Francisco Sousa; Jelena Dinic; Suvi Holmstedt; Amílcar Roberto; Ana Díaz-Lanza; Catarina P Reis; Milica Pesic; Nuno R Candeias; Ricardo J Ferreira; Noélia Duarte; Carlos A M Afonso; Patrícia Rijo Journal: Front Pharmacol Date: 2020-11-17 Impact factor: 5.810
Authors: Rosaria Acquaviva; Giuseppe A Malfa; Monica R Loizzo; Jianbo Xiao; Simone Bianchi; Rosa Tundis Journal: Molecules Date: 2022-07-26 Impact factor: 4.927