Lee Kellingray1, Gwénaëlle Le Gall2, Marianne Defernez3, Ian L P Beales4, Ngozi Franslem-Elumogo5, Arjan Narbad6. 1. Gut Health and Microbiome Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UA, UK; NIHR Health Protection Research Unit in Gastrointestinal Infections, UK. Electronic address: lee.kellingray@quadram.ac.uk. 2. Analytical Sciences Unit, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UA, UK. Electronic address: gwenaelle.legall@quadram.ac.uk. 3. Analytical Sciences Unit, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UA, UK. Electronic address: marianne.defernez@quadram.ac.uk. 4. Gastroenterology, Norfolk and Norwich University Hospital, Norwich NR4 7UY, UK. Electronic address: ian.beales@nnuh.nhs.uk. 5. Microbiology, Norfolk and Norwich University Hospital, Norwich NR4 7UY, UK. Electronic address: ngozi.elumogo@nnuh.nhs.uk. 6. Gut Health and Microbiome Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UA, UK; NIHR Health Protection Research Unit in Gastrointestinal Infections, UK. Electronic address: arjan.narbad@quadram.ac.uk.
Abstract
OBJECTIVES: This study aimed to examine changes to the microbiota composition and metabolic profiles of seven patients with recurrent Clostridium difficile infection (rCDI), following treatment with faecal microbiota transplant (FMT). METHODS: 16S rDNA sequencing and 1H NMR were performed on faecal samples from the patients (pre-, post-FMT, and follow-up) and the associated donor samples. Sparse partial-least-square analysis was used to identify correlations between the two datasets. RESULTS: The patients' microbiota post-FMT tended to shift towards the donor microbiota, specifically through proportional increases of Bacteroides, Blautia, and Ruminococcus, and proportional decreases of Enterococcus, Escherichia, and Klebsiella. However, although cured of infection, one patient, who suffers from chronic alcohol abuse, retained the compositional characteristics of the pre-FMT microbiota. Following FMT, increased levels of short-chain fatty acids, particularly butyrate and acetate, were observed in all patients. Sparse partial-least-square analysis confirmed a positive correlation between butyrate and Bacteroides, Blautia, and Ruminococcus, with a negative correlation between butyrate and Klebsiella and Enterococcus. CONCLUSIONS: Clear differences were observed in the microbiota composition and metabolic profiles between donors and rCDI patients, which were largely resolved in patients following FMT. Increased levels of butyrate appear to be a factor associated with resolution of rCDI.
OBJECTIVES: This study aimed to examine changes to the microbiota composition and metabolic profiles of seven patients with recurrent Clostridium difficileinfection (rCDI), following treatment with faecal microbiota transplant (FMT). METHODS: 16S rDNA sequencing and 1H NMR were performed on faecal samples from the patients (pre-, post-FMT, and follow-up) and the associated donor samples. Sparse partial-least-square analysis was used to identify correlations between the two datasets. RESULTS: The patients' microbiota post-FMT tended to shift towards the donor microbiota, specifically through proportional increases of Bacteroides, Blautia, and Ruminococcus, and proportional decreases of Enterococcus, Escherichia, and Klebsiella. However, although cured of infection, one patient, who suffers from chronic alcohol abuse, retained the compositional characteristics of the pre-FMT microbiota. Following FMT, increased levels of short-chain fatty acids, particularly butyrate and acetate, were observed in all patients. Sparse partial-least-square analysis confirmed a positive correlation between butyrate and Bacteroides, Blautia, and Ruminococcus, with a negative correlation between butyrate and Klebsiella and Enterococcus. CONCLUSIONS: Clear differences were observed in the microbiota composition and metabolic profiles between donors and rCDI patients, which were largely resolved in patients following FMT. Increased levels of butyrate appear to be a factor associated with resolution of rCDI.
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