Literature DB >> 29745378

Correction to: The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation.

A Herrmann1, S Wittmann1, D Thomas2, C N Shepard3, B Kim3,4, N Ferreirós2, T Gramberg1.   

Abstract

[This corrects the article DOI: 10.1186/s13100-018-0116-5.].

Entities:  

Year:  2018        PMID: 29745378      PMCID: PMC5930422          DOI: 10.1186/s13100-018-0121-8

Source DB:  PubMed          Journal:  Mob DNA


Correction

The original article [1] contains an omission in the Methods. The authors would like to note that the paragraph constituting the L1 promoter assay should read as the following: L1 promoter assay HEK-293T cells were transfected with the L1 promoter luciferase reporter plasmid (L1RP-luc) together with empty vector (pcDNA6mycHis) or SAMHD1 expressing vector using calcium phosphate. The reporter plasmid L1RP-luc and the L1 promoter activity assay have been described previously [2]. Two days posttransfection, cells were lysed with Cell Culture Lysis 5× reagent (Promega) and luciferase activity was quantified using commercially available components (Promega).
  2 in total

1.  Methyl-CpG-binding protein 2 represses LINE-1 expression and retrotransposition but not Alu transcription.

Authors:  F Yu; N Zingler; G Schumann; W H Strätling
Journal:  Nucleic Acids Res       Date:  2001-11-01       Impact factor: 16.971

2.  The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation.

Authors:  Alexandra Herrmann; Sabine Wittmann; Dominique Thomas; Caitlin N Shepard; Baek Kim; Nerea Ferreirós; Thomas Gramberg
Journal:  Mob DNA       Date:  2018-03-28
  2 in total

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