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Literature DB >> 29742306

A Defined and Flexible Pocket Explains Aryl Substrate Promiscuity of the Cahuitamycin Starter Unit-Activating Enzyme CahJ.

Ashootosh Tripathi^1,2, Sung Ryeol Park^1,3, Andrew P Sikkema^1,4,5, Hyo Je Cho⁶, Jianfeng Wu⁷, Brian Lee¹, Chuanwu Xi⁷, Janet L Smith^1,4, David H Sherman^1,2,8,9.

Abstract

Cahuitamycins are biofilm inhibitors assembled by a convergent nonribosomal peptide synthetase pathway. Previous genetic analysis indicated that a discrete enzyme, CahJ, serves as a gatekeeper for cahuitamycin structural diversification. Here, the CahJ protein was probed structurally and functionally to guide the formation of new analogues by mutasynthetic studies. This analysis enabled the in vivo production of a new cahuitamycin congener through targeted precursor incorporation.

Entities: CellLine Chemical Disease Species

Keywords: adenylation domains; biosynthesis; kinetics; natural products; protein structures

Mesh：

Substances：

Year: 2018 PMID： 29742306 PMCID： PMC6105470 DOI： 10.1002/cbic.201800233

Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164

18 in total

Review 1. Natural Products as Sources of New Drugs from 1981 to 2014.

Authors: David J Newman; Gordon M Cragg
Journal: J Nat Prod Date: 2016-02-07 Impact factor: 4.050

2. The nonribosomal peptide synthetase HMWP2 forms a thiazoline ring during biogenesis of yersiniabactin, an iron-chelating virulence factor of yersinia pestis

Authors:
Journal: Biochemistry Date: 1998-12-01 Impact factor: 3.162

3. Structural and functional investigation of the intermolecular interaction between NRPS adenylation and carrier protein domains.

Authors: Jesse A Sundlov; Ce Shi; Daniel J Wilson; Courtney C Aldrich; Andrew M Gulick
Journal: Chem Biol Date: 2012-02-24

4. Characterization of the formation of the pyrrole moiety during clorobiocin and coumermycin A1 biosynthesis.

Authors: Sylvie Garneau; Pieter C Dorrestein; Neil L Kelleher; Christopher T Walsh
Journal: Biochemistry Date: 2005-03-01 Impact factor: 3.162

5. Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate product.

Authors: F Rusnak; W S Faraci; C T Walsh
Journal: Biochemistry Date: 1989-08-22 Impact factor: 3.162

6. Biochemical and structural characterization of bisubstrate inhibitors of BasE, the self-standing nonribosomal peptide synthetase adenylate-forming enzyme of acinetobactin synthesis.

Authors: Eric J Drake; Benjamin P Duckworth; João Neres; Courtney C Aldrich; Andrew M Gulick
Journal: Biochemistry Date: 2010-11-02 Impact factor: 3.162

7. Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin.

Authors: L E Quadri; J Sello; T A Keating; P H Weinreb; C T Walsh
Journal: Chem Biol Date: 1998-11

Review 8. Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase.

Authors: Andrew M Gulick
Journal: ACS Chem Biol Date: 2009-10-16 Impact factor: 5.100

9. Chain initiation in the leinamycin-producing hybrid nonribosomal peptide/polyketide synthetase from Streptomyces atroolivaceus S-140. Discrete, monofunctional adenylation enzyme and peptidyl carrier protein that directly load D-alanine.

Authors: Gong-Li Tang; Yi-Qiang Cheng; Ben Shen
Journal: J Biol Chem Date: 2007-05-14 Impact factor: 5.157