OBJECTIVE: To study the effects of intrahippocampal injection of cellular prion protein (PrPC) antibody on cognitive deficits of APPswe/PSEN1dE9 transgenic mice. METHODS: Eight-month-old male APPswe/PSEN1dE9 transgenic mice were subjected to bilateral intrahippocampal injection of a single dose (2 µL) of anti-PrPC monoclonal antibody (EP1802Y) or PBS, with wild-type C57Bl/6J mice serving as the control group. After two months, the mice were tested for cognitive behaviors using open filed (OF) test, Morris water maze (MWM) test, fear conditioning (FC) test, and novel object recognition (NOR) test, and immunohistochemistry was used to examine the changes in hippocampal expression of Aβ1-42. RESULTS: The EP1802Y-treated and PBS-treated mice showed no significantly differences in the performance in OF test in terms of central activity time or total distance of activity (P>0.05), nor in NOR test in terms of novel object recognition index (P>0.05). In MWM test, the EP1802Y-treated and PBS-treated mice showed significantly reduced crossings of the hidden platform as compared with the wild-type mice (P<0.05), but EP1802Y-treated mice had a significantly shorter swimming distance to find the platform than PBS-treated mice (P<0.05). No significant differences were found in the results of FC test among the 3 groups. Immunohistochemistry revealed a significantly reduced expression of Aβ1-42 in the hippocampus of EP1802Y-treated mice. CONCLUSION: Intrahippocampal injection of PrPC antibody can improve cognitive deficits of APPswe/PSEN1dE9 transgenic mice, which sheds light on a novel therapeutic approach for Alzheimer's disease that targets PrPC to lower the toxicity of Aβ oligomer.
OBJECTIVE: To study the effects of intrahippocampal injection of cellular prion protein (PrPC) antibody on cognitive deficits of APPswe/PSEN1dE9 transgenic mice. METHODS: Eight-month-old male APPswe/PSEN1dE9 transgenic mice were subjected to bilateral intrahippocampal injection of a single dose (2 µL) of anti-PrPC monoclonal antibody (EP1802Y) or PBS, with wild-type C57Bl/6J mice serving as the control group. After two months, the mice were tested for cognitive behaviors using open filed (OF) test, Morris water maze (MWM) test, fear conditioning (FC) test, and novel object recognition (NOR) test, and immunohistochemistry was used to examine the changes in hippocampal expression of Aβ1-42. RESULTS: The EP1802Y-treated and PBS-treated mice showed no significantly differences in the performance in OF test in terms of central activity time or total distance of activity (P>0.05), nor in NOR test in terms of novel object recognition index (P>0.05). In MWM test, the EP1802Y-treated and PBS-treated mice showed significantly reduced crossings of the hidden platform as compared with the wild-type mice (P<0.05), but EP1802Y-treated mice had a significantly shorter swimming distance to find the platform than PBS-treated mice (P<0.05). No significant differences were found in the results of FC test among the 3 groups. Immunohistochemistry revealed a significantly reduced expression of Aβ1-42 in the hippocampus of EP1802Y-treated mice. CONCLUSION: Intrahippocampal injection of PrPC antibody can improve cognitive deficits of APPswe/PSEN1dE9 transgenic mice, which sheds light on a novel therapeutic approach for Alzheimer's disease that targets PrPC to lower the toxicity of Aβ oligomer.
Authors: Clemens Falker; Alexander Hartmann; Inga Guett; Frank Dohler; Hermann Altmeppen; Christian Betzel; Robin Schubert; Dana Thurm; Florian Wegwitz; Pooja Joshi; Claudia Verderio; Susanne Krasemann; Markus Glatzel Journal: J Neurochem Date: 2016-03-02 Impact factor: 5.372
Authors: Shanna L Burke; Miriam J Rodriguez; Warren Barker; Maria T Greig-Custo; Monica Rosselli; David A Loewenstein; Ranjan Duara Journal: J Int Neuropsychol Soc Date: 2017-09-18 Impact factor: 2.892
Authors: Mikhail A Kostylev; Adam C Kaufman; Haakon B Nygaard; Pujan Patel; Laura T Haas; Erik C Gunther; Alexander Vortmeyer; Stephen M Strittmatter Journal: J Biol Chem Date: 2015-05-27 Impact factor: 5.157
Authors: Darragh B Freir; Andrew J Nicoll; Igor Klyubin; Silvia Panico; Jessica M Mc Donald; Emmanuel Risse; Emmanuel A Asante; Mark A Farrow; Richard B Sessions; Helen R Saibil; Anthony R Clarke; Michael J Rowan; Dominic M Walsh; John Collinge Journal: Nat Commun Date: 2011-06-07 Impact factor: 14.919