Ecotoxicity of the two veterinarian antibiotics ceftiofur and cefapirin before and after photo-transformation.

The release of antibiotics into the environment may lead to deleterious effects in non-target organisms as well as pressure in antimicrobial resistance acquirement. Ceftiofur (CEF) and cefapirin (CEPA) are veterinary cephalosporins used for recurrent and economically relevant infections. Both antibiotics have been detected in aquatic environments and their fate during drinking water processing is still unknown. This work investigated the acute and chronic toxicities of CEF and CEPA towards aquatic organisms including stability tests. Complementary, the effects of water disinfection radiation (UV-C, 254nm) on ecotoxicological responses were studied. CEF and CEPA have significant decay during Daphnia magna tests, portraying half-lives (t1/2) of 49 and 53h, respectively. During tests with green algae (Scenedesmus spec.), CEPA was more instable (t1/2 88h) than CEF (t1/2 267h). CEF and its presumable hydrolysis products induced deleterious effects in Daphnia magna (48h EC50 139, LC50 179 in μM), which was not observed with Scenedesmus spec. (72h NOAEC 82.5±2.5μM). In the case of CEPA, no toxic effects were observed in either test (48h EC-LC50>510 and 72h NOAEC 57±6, in μM). Photolysis of CEPA resulted in toxic products, which were effective for the cladoceran but not for the green algae. On the other hand, the different radiation doses studied did not affect CEF ecotoxicity. This investigation illustrates the importance of cephalosporin hydrolysis during standard toxicity tests. Furthermore, the potential formation of species-specific toxic compounds during water processing is demonstrated, highlighting the need of further assessing toxicity of both cephalosporins and their transformation products.