María Virginia Ozcariz-Fermoselle1, Raúl Fraile-Fabero2, Tomás Girbés-Juan3, Oscar Arce-Cervantes4, Juan Andrés Oria de Rueda-Salgueiro5, Anabela Marisa Azul6. 1. Cátedra de Micología, Escuela Técnica Superior de Ingenierías Agrarias, Campus Palencia, Universidad de Valladolid, Avenida de Madrid, 57, 34004 Palencia, Spain. Electronic address: mvirginiaozcariz@gmail.com. 2. Cátedra de Micología, Escuela Técnica Superior de Ingenierías Agrarias, Campus Palencia, Universidad de Valladolid, Avenida de Madrid, 57, 34004 Palencia, Spain; Nutrición y Bromatología, Facultad de Medicina, Universidad de Valladolid, Av. Ramón y Cajal n° 7, 47003 Valladolid, Spain. 3. Nutrición y Bromatología, Facultad de Medicina, Universidad de Valladolid, Av. Ramón y Cajal n° 7, 47003 Valladolid, Spain. 4. Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km1, 43600 Tulancingo, Hgo, Mexico. 5. Cátedra de Micología, Escuela Técnica Superior de Ingenierías Agrarias, Campus Palencia, Universidad de Valladolid, Avenida de Madrid, 57, 34004 Palencia, Spain. 6. Center for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, Rua Larga, Pólo I, 1st Floor, 3004-504 Coimbra, Portugal.
Abstract
BACKGROUND: The wastes of pecan nut (Carya illinoinensis (Wangenh.) K. Koch) production are increasing worldwide and have high concentrations of tannins and phenols. AIMS: To study the biodegradation of lignocellulosic wastes of pecan used as solid substrate for the cultivation of the white-rot fungus Ganoderma lucidum (Curtis) P. Karst. METHODS: Six formulations of pecan wastes were used as solid substrate: pecan shells (PS100), pecan pericarp (PP100), pecan wood-chips (PB100), and the combinations PS50+PP50, PB50+PS50 and PB50+PP50. The substrates were inoculated with a wild strain of G. lucidum collected in the Iberian Peninsula. The biodegradation capability of G. lucidum was estimated by using the mycelial growth rate, the biological efficiency, the production and the dry biological efficiency. RESULTS: Notably, all solid substrates were suitable for G. lucidum growth and mushroom yield. The best performance in mushroom yield was obtained with PB100 (55.4% BE), followed by PB50+PP50 (31.7% BE) and PB50+PS50 (25.4% BE). The mushroom yield in the substrates containing pecan wood-chips (PB) was significantly higher. CONCLUSIONS: Our study is leading the way in attempting the cultivation of G. lucidum on lignocellulosic pecan waste. These results show an environmentally friendly alternative that increases the benefits for the global pecan industry, especially in rural areas, and transforms biomass into mushrooms with nutraceutical properties and biotechnological applications.
BACKGROUND: The wastes of pecan nut (Carya illinoinensis (Wangenh.) K. Koch) production are increasing worldwide and have high concentrations of tannins and phenols. AIMS: To study the biodegradation of lignocellulosic wastes of pecan used as solid substrate for the cultivation of the white-rot fungus Ganoderma lucidum (Curtis) P. Karst. METHODS: Six formulations of pecan wastes were used as solid substrate: pecan shells (PS100), pecan pericarp (PP100), pecan wood-chips (PB100), and the combinations PS50+PP50, PB50+PS50 and PB50+PP50. The substrates were inoculated with a wild strain of G. lucidum collected in the Iberian Peninsula. The biodegradation capability of G. lucidum was estimated by using the mycelial growth rate, the biological efficiency, the production and the dry biological efficiency. RESULTS: Notably, all solid substrates were suitable for G. lucidum growth and mushroom yield. The best performance in mushroom yield was obtained with PB100 (55.4% BE), followed by PB50+PP50 (31.7% BE) and PB50+PS50 (25.4% BE). The mushroom yield in the substrates containing pecan wood-chips (PB) was significantly higher. CONCLUSIONS: Our study is leading the way in attempting the cultivation of G. lucidum on lignocellulosic pecan waste. These results show an environmentally friendly alternative that increases the benefits for the global pecan industry, especially in rural areas, and transforms biomass into mushrooms with nutraceutical properties and biotechnological applications.