Carlos Fernando de Almeida Barros Mourão1,2, Emanuelle Stellet Lourenço1, Jhonathan Raphael Barros Nascimento1, Rafael Coutinho Mello Machado1, Alexandre Malta Rossi3, Paulo Emílio Corrêa Leite4, José Mauro Granjeiro5,6, Gutemberg Gomes Alves2, Mônica Diuana Calasans-Maia7. 1. Graduate Program in Dentistry, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil. 2. Department of Molecular and Cell Biology, Institute of Biology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil. 3. Biomaterials Laboratory, Brazilian Center for Physical Studies, Rio de Janeiro, Brazil. 4. Laboratory of Ultrastructure and Cellular Biology Hertha Meyer, Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. 5. Nacional Institute of Metrology, Quality and Technology, Duque de Caxias, Rio de Janeiro, Brazil. 6. Dentistry School, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil. 7. Oral Surgery Department, Dentistry School, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil. monicacalasansmaia@gmail.com.
Abstract
OBJECTIVE: The combination of calcium phosphate with blood-derived growth factors (BDGF) has been widely used in bone regeneration procedures although its benefits are still unclear. The purpose of this study was to evaluate whether or not BDGF improves the efficacy of a modified carbonated calcium phosphate biomaterial in sinus floor augmentation. MATERIAL AND METHODS:Ten patients underwent 20 sinus floor augmentation procedures usingnanostructured carbonated hydroxyapatite (cHA) microspheres alone or associated with BDGF in a randomized controlled clinical trial. The in vitro release of growth factors was assessed by an elution assay. Bone grafts were randomly implanted in the right and left maxillary sinuses of each participant, associated either with a 0.9% saline solution or BDGF. Bone gain was evaluated through cone beam tomography after 180 days. RESULTS:Nine women and one man composed the sample. The blood-derived concentrates were able to release high levels of growth factors and cytokines. A significant clinical advantage was observed in the use of the BDGF after fibrin polymerization around the biomaterial microspheres, optimizing the surgical procedures, thereby reducing the time and displacement, and improving the adaptation of the biomaterial in the maxillary sinus. No synergistic effect was observed in bone formation when cHA was associated with BDGF (p > 0.05). CONCLUSION: Equivalent new bone formation was observed for cHA in the presence or absence of the BDGF concentrate in bilateral sinus floor elevation after 6 months. Blood-derived growth factors did not improve bone repair when associated with calcium phosphate in sinus lift procedures.
RCT Entities:
OBJECTIVE: The combination of calcium phosphate with blood-derived growth factors (BDGF) has been widely used in bone regeneration procedures although its benefits are still unclear. The purpose of this study was to evaluate whether or not BDGF improves the efficacy of a modified carbonated calcium phosphate biomaterial in sinus floor augmentation. MATERIAL AND METHODS: Ten patients underwent 20 sinus floor augmentation procedures using nanostructured carbonated hydroxyapatite (cHA) microspheres alone or associated with BDGF in a randomized controlled clinical trial. The in vitro release of growth factors was assessed by an elution assay. Bone grafts were randomly implanted in the right and left maxillary sinuses of each participant, associated either with a 0.9% saline solution or BDGF. Bone gain was evaluated through cone beam tomography after 180 days. RESULTS: Nine women and one man composed the sample. The blood-derived concentrates were able to release high levels of growth factors and cytokines. A significant clinical advantage was observed in the use of the BDGF after fibrin polymerization around the biomaterial microspheres, optimizing the surgical procedures, thereby reducing the time and displacement, and improving the adaptation of the biomaterial in the maxillary sinus. No synergistic effect was observed in bone formation when cHA was associated with BDGF (p > 0.05). CONCLUSION: Equivalent new bone formation was observed for cHA in the presence or absence of the BDGF concentrate in bilateral sinus floor elevation after 6 months. Blood-derived growth factors did not improve bone repair when associated with calcium phosphate in sinus lift procedures.
Authors: Rodrigo F B Resende; Suelen C Sartoretto; Marcelo J Uzeda; Adriana T N N Alves; José A Calasans-Maia; Alexandre M Rossi; José Mauro Granjeiro; Mônica D Calasans-Maia Journal: Materials (Basel) Date: 2019-11-06 Impact factor: 3.623
Authors: Thiago Schneider Werner Vianna; Suelen Cristina Sartoretto; Adriana Terezinha Neves Novellino Alves; Rodrigo Figueiredo de Brito Resende; Carlos Fernando de Almeida Barros Mourão; Jose de Albuquerque Calasans-Maia; Victor R Martinez-Zelaya; Alexandre Malta Rossi; Jose Mauro Granjeiro; Monica Diuana Calasans-Maia; Rafael Seabra Louro Journal: J Funct Biomater Date: 2020-12-04