Xiao Jiang1, Junhao Yan1, Yan Sheng1, Mei Sun1, Linlin Cui2, Zi-Jiang Chen3. 1. Centre for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250001, China; The Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, 250001, China; National Research Centre for Assisted Reproductive Technology and Reproductive Genetics, Jinan, 250001, China. 2. Centre for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250001, China; The Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, 250001, China; National Research Centre for Assisted Reproductive Technology and Reproductive Genetics, Jinan, 250001, China. Electronic address: fdclear3@126.com. 3. Centre for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250001, China; The Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, 250001, China; National Research Centre for Assisted Reproductive Technology and Reproductive Genetics, Jinan, 250001, China; Centre for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200000, China; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, 200000, China. Electronic address: chenzijiang@hotmail.com.
Abstract
RESEARCH QUESTION: Does an association exist between serum anti-Müllerian hormone (AMH) level, the marker of biological ovarian age, and embryonic aneuploidy risk in recurrent spontaneous miscarriage (RSM) patients of reproductive age? DESIGN: This retrospective study included a total of 422 IVF cycles of 394 unexplained RSM patients undergoing preimplantation genetic testing for aneuploidy (PGT-A), enrolled from January 2014 to December 2016. Subjects were divided into three groups according to the 25th (1.50 ng/ml) and 75th (5.60 ng/ml) percentiles of AMH level (Group 1: low AMH <1.50 ng/ml [N = 107], Group 2: normal AMH 1.50- < 5.60 ng/ml [N = 210] and Group 3: high AMH ≥ 5.60 ng/ml [N = 105]). RESULTS: There was a significant difference in embryonic aneuploid rate between AMH groups (66.7% versus 42.9% versus 50.0%, Groups 1 to 3, respectively, P = 0.006). It was significantly higher in the low AMH group (Group 1) compared with that in the normal AMH group (Group 2, P1vs2 = 0.002) and high AMH group (Group 3, P1vs3 = 0.015). After age stratification, embryonic aneuploidy rate was still significantly different among AMH groups with a similar trend in women ≥35 years old (68.2% versus 54.4% versus 51.0%, P = 0.038, P1vs2 = 0.025, P1vs3 = 0.035), but not in young subjects. CONCLUSIONS: These findings indicate that low AMH level was associated with increased risk of embryo aneuploidy only in women of advanced age. Maternal diminished ovarian reserve along with oocyte ageing may contribute to impaired chromosomal competence of the embryo.
RESEARCH QUESTION: Does an association exist between serum anti-Müllerian hormone (AMH) level, the marker of biological ovarian age, and embryonic aneuploidy risk in recurrent spontaneous miscarriage (RSM) patients of reproductive age? DESIGN: This retrospective study included a total of 422 IVF cycles of 394 unexplained RSM patients undergoing preimplantation genetic testing for aneuploidy (PGT-A), enrolled from January 2014 to December 2016. Subjects were divided into three groups according to the 25th (1.50 ng/ml) and 75th (5.60 ng/ml) percentiles of AMH level (Group 1: low AMH <1.50 ng/ml [N = 107], Group 2: normal AMH 1.50- < 5.60 ng/ml [N = 210] and Group 3: high AMH ≥ 5.60 ng/ml [N = 105]). RESULTS: There was a significant difference in embryonic aneuploid rate between AMH groups (66.7% versus 42.9% versus 50.0%, Groups 1 to 3, respectively, P = 0.006). It was significantly higher in the low AMH group (Group 1) compared with that in the normal AMH group (Group 2, P1vs2 = 0.002) and high AMH group (Group 3, P1vs3 = 0.015). After age stratification, embryonic aneuploidy rate was still significantly different among AMH groups with a similar trend in women ≥35 years old (68.2% versus 54.4% versus 51.0%, P = 0.038, P1vs2 = 0.025, P1vs3 = 0.035), but not in young subjects. CONCLUSIONS: These findings indicate that low AMH level was associated with increased risk of embryo aneuploidy only in women of advanced age. Maternal diminished ovarian reserve along with oocyte ageing may contribute to impaired chromosomal competence of the embryo.
Authors: D Levenson; R Romero; V Garcia-Flores; D Miller; Y Xu; A Sahi; S S Hassan; N Gomez-Lopez Journal: Clin Exp Immunol Date: 2020-05-07 Impact factor: 4.330
Authors: Ermanno Greco; Katarzyna Litwicka; Maria Giulia Minasi; Elisabetta Cursio; Pier Francesco Greco; Paolo Barillari Journal: Int J Mol Sci Date: 2020-06-19 Impact factor: 5.923