| Literature DB >> 29722289 |
Yonglin Wang1, Xiaoping Hu2, Yulin Fang1, Amy Anchieta3, Polly H Goldman3, Gustavo Hernandez3, Steven J Klosterman3.
Abstract
Entities:
Keywords: DHN; fungi; melanin; pathogenicity; pigment; regulation
Year: 2018 PMID: 29722289 PMCID: PMC7008214 DOI: 10.1099/mic.0.000661
Source DB: PubMed Journal: Microbiology ISSN: 1350-0872 Impact factor: 2.777
Fig. 6.Schematic diagram depicting VdCmr1-dependent DHN melanogenesis in V. dahliae. Key proteins involving the DHN melanogenesis pathway are depicted from the initial acetyl-CoA or malonyl-CoA precursors through production of DHN melanin [4]. The Ssk2-Pbs2-Hog1 signalling cascade is a regulator of melanin biosynthesis in V. dahliae [14]. The transcription factor VdMcm1 and the polyketide chain shortening enzyme Vayg1 also modulate DHN melanogenesis. Our work identified several key genes that contribute to DHN melanin biosynthesis and that are regulated by VdCmr1. Some of these genes are found within the cluster enriched in DHN melanin biosynthetic genes within the V. dahliae genome. The non-melanin synthesis-related genes, up or downregulated during microsclerotial development [7], are regulated by VdCmr1 as demonstrated in this study; these genes VDAG_03079 (catalase) and VDAG_08741 VDECH (endochitinase, VDAG_08741) also reside outside of the gene cluster enriched in melanin biosynthetic genes. aVDAG_03665 may catalyse both T4HN and T3HN reductase steps based upon the strong homology of VDAG_03665 to the THR1 protein of Colletotrichum lagenarium, which can catalyse both T4HN and T3HN reductase steps [41].