Literature DB >> 29721992

Photoreceptor Outer Segment Isolation from a Single Canine Retina for RPE Phagocytosis Assay.

Raghavi Sudharsan1, Michael H Elliott2, Natalia Dolgova1, Gustavo D Aguirre1, William A Beltran3.   

Abstract

Protocols for photoreceptor outer segment (POS) isolation that can be used in phagocytosis assays of retinal pigment epithelium (RPE) cells have routinely used a large number of cow or pig eyes. However, when working with large animal models (e.g., dog, cats, transgenic pigs) of inherited retinal degenerative diseases, access to retinal tissues may be limited. An optimized protocol is presented in this paper to isolate sufficient POS from a single canine retina for use in RPE phagocytosis assays.

Entities:  

Keywords:  Canine; Inherited retinal degeneration; Phagocytosis; Photoreceptor outer segment isolation; Retinal pigment epithelium

Mesh：

Substances：

Year:  2018        PMID： 29721992      PMCID： PMC6048966          DOI： 10.1007/978-3-319-75402-4_72

Source DB:  PubMed          Journal:  Adv Exp Med Biol        ISSN： 0065-2598            Impact factor:   2.622


  10 in total

Review 1.  Bestrophins and retinopathies.

Authors:  Qinghuan Xiao; H Criss Hartzell; Kuai Yu
Journal:  Pflugers Arch       Date:  2010-03-28       Impact factor: 3.657

2.  Mutations in RPE65 cause autosomal recessive childhood-onset severe retinal dystrophy.

Authors:  S M Gu; D A Thompson; C R Srikumari; B Lorenz; U Finckh; A Nicoletti; K R Murthy; M Rathmann; G Kumaramanickavel; M J Denton; A Gal
Journal:  Nat Genet       Date:  1997-10       Impact factor: 38.330

Review 3.  The retinal pigment epithelium in visual function.

Authors:  Olaf Strauss
Journal:  Physiol Rev       Date:  2005-07       Impact factor: 37.312

4.  Mutations in RPE65 cause Leber's congenital amaurosis.

Authors:  F Marlhens; C Bareil; J M Griffoin; E Zrenner; P Amalric; C Eliaou; S Y Liu; E Harris; T M Redmond; B Arnaud; M Claustres; C P Hamel
Journal:  Nat Genet       Date:  1997-10       Impact factor: 38.330

5.  Detailed characterization of the lipid composition of detergent-resistant membranes from photoreceptor rod outer segment membranes.

Authors:  Rex E Martin; Michael H Elliott; Richard S Brush; Robert E Anderson
Journal:  Invest Ophthalmol Vis Sci       Date:  2005-04       Impact factor: 4.799

6.  Beta-glucuronidase mediated pathway essential for retinal pigment epithelial degradation of glycosaminoglycans. Disease expression and in vitro disease correction using retroviral mediated cDNA transfer.

Authors:  L E Stramm; J H Wolfe; E H Schuchman; M E Haskins; D F Patterson; G D Aguirre
Journal:  Exp Eye Res       Date:  1990-05       Impact factor: 3.467

7.  Analysis of photoreceptor outer segment phagocytosis by RPE cells in culture.

Authors:  Yingyu Mao; Silvia C Finnemann
Journal:  Methods Mol Biol       Date:  2013

8.  Congenital stationary night blindness in the dog: common mutation in the RPE65 gene indicates founder effect.

Authors:  G D Aguirre; V Baldwin; S Pearce-Kelling; K Narfström; K Ray; G M Acland
Journal:  Mol Vis       Date:  1998-10-30       Impact factor: 2.367

9.  Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease.

Authors:  Karina E Guziewicz; Barbara Zangerl; Sarah J Lindauer; Robert F Mullins; Lynne S Sandmeyer; Bruce H Grahn; Edwin M Stone; Gregory M Acland; Gustavo D Aguirre
Journal:  Invest Ophthalmol Vis Sci       Date:  2007-05       Impact factor: 4.799

10.  Tissue culture of cat retinal pigment epithelium.

Authors:  L E Stramm; M E Haskins; M M McGovern; G D Aguirre
Journal:  Exp Eye Res       Date:  1983-01       Impact factor: 3.467
  10 in total
  1 in total

1.  Loss of the Bardet-Biedl protein Bbs1 alters photoreceptor outer segment protein and lipid composition.

Authors:  Markus Masek; Christelle Etard; Uwe Strähle; Ruxandra Bachmann-Gagescu; Claudia Hofmann; Andreas J Hülsmeier; Jingjing Zang; Masanari Takamiya; Matthias Gesemann; Stephan C F Neuhauss; Thorsten Hornemann
Journal:  Nat Commun       Date:  2022-03-11       Impact factor: 14.919
  1 in total

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