| Literature DB >> 29720184 |
Lemu Golassa1,2, Berhanu Erko3, Frederick N Baliraine4, Abraham Aseffa5, Göte Swedberg6.
Abstract
After publication of the original article [1], it came to the authors' attention that the primers mentioned in Table 1 for the amplification of the pvcrt-o gene of Plasmodium vivax are not the ones actually used for the experiments. The correct primers and PCR product size are as below.Entities:
Year: 2018 PMID: 29720184 PMCID: PMC5932851 DOI: 10.1186/s12936-018-2338-x
Source DB: PubMed Journal: Malar J ISSN: 1475-2875 Impact factor: 2.979
Primers used for amplifications of pvcrt-o and pvmdr-1 marker genes
| PCR | Sequences 5´ → 3´ |
|---|---|
| Outer forward (OF) | CCA GTG AGA AGC CCC TGT TC |
| Outer reverse (OR) | CGA AGT ATG CGC GTG AAG |
| Nested forward (NF) | AAG AGC CGT CAA GCC ATC |
| Nested reverse (NR) | AGT TTC CCT CTA CAC CCG |
| GGG GAC GTC CTC TTG TAT TT |
OF Outer forward, OR outer reverse, NF nested forward, NR nested reverse. Size (nested) 1190 bp