Literature DB >> 29720184

Correction to: Polymorphisms in chloroquine resistance-associated genes in Plasmodium vivax in Ethiopia.

Lemu Golassa1,2, Berhanu Erko3, Frederick N Baliraine4, Abraham Aseffa5, Göte Swedberg6.   

Abstract

After publication of the original article [1], it came to the authors' attention that the primers mentioned in Table 1 for the amplification of the pvcrt-o gene of Plasmodium vivax are not the ones actually used for the experiments. The correct primers and PCR product size are as below.

Entities:  

Year:  2018        PMID: 29720184      PMCID: PMC5932851          DOI: 10.1186/s12936-018-2338-x

Source DB:  PubMed          Journal:  Malar J        ISSN: 1475-2875            Impact factor:   2.979


Correction to: Malaria J (2015) 14:164 10.1186/s12936-015-0625-3

After publication of the original article [1], it came to the authors’ attention that the primers mentioned in Table 1 for the amplification of the pvcrt-o gene of Plasmodium vivax are not the ones actually used for the experiments. The correct primers and PCR product size are as below:
Table 1

Primers used for amplifications of pvcrt-o and pvmdr-1 marker genes

PCRSequences 5´ → 3´
Outer forward (OF)CCA GTG AGA AGC CCC TGT TC
Outer reverse (OR)CGA AGT ATG CGC GTG AAG
Nested forward (NF)AAG AGC CGT CAA GCC ATC
Nested reverse (NR)AGT TTC CCT CTA CAC CCG
pvcrt-o (Rseq)GGG GAC GTC CTC TTG TAT TT

OF Outer forward, OR outer reverse, NF nested forward, NR nested reverse. Size (nested) 1190 bp

Primers used for amplifications of pvcrt-o and pvmdr-1 marker genes OF Outer forward, OR outer reverse, NF nested forward, NR nested reverse. Size (nested) 1190 bp
  1 in total

1.  Polymorphisms in chloroquine resistance-associated genes in Plasmodium vivax in Ethiopia.

Authors:  Lemu Golassa; Berhanu Erko; Frederick N Baliraine; Abraham Aseffa; Göte Swedberg
Journal:  Malar J       Date:  2015-04-16       Impact factor: 2.979

  1 in total

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