S H Yang1, W H Chen1, E T Wang2, W F Chen1, J Yan3, X Z Han3, C F Tian1, X H Sui1, R P Singh4, G M Jiang5, W X Chen1. 1. State Key Laboratory of Agrobiotechnology, College of Biological Sciences and Rhizobium Research Center, China Agricultural University, Beijing, China. 2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, Distrito Federal, México. 3. Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, Heilongjiang Province, China. 4. Microbial Genomics Laboratory, National Bureau of Agriculturally Important Micro-organisms, Maunath Bhanjan, Uttar Pradesh, India. 5. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, The Chinese Academy of Sciences, Beijing, China.
Abstract
AIMS: The aim of the study was to survey rhizobial biogeography and to inoculate soybean with selected rhizobia in China to enhance symbiotic nitrogen fixation (SNF). METHODS AND RESULTS: Biogeography, genetic diversity and phylogeny of soybean rhizobia were surveyed. Inocula were prepared and applied to soybean. Results showed that Bradyrhizobium elkanii and Ensifer fredii were widely distributed in acid and alkaline soils respectively. Available iron was detected as the first determinant for distribution of the two rhizobia and the soybean varieties did not greatly affect the rhizobial compatibility. Geographical latitude and precipitation in June were the main geographical and climatic factors affecting the rhizobial distribution. Inoculation with selected rhizobia increased the nodule number, fresh weight, occupation ratio, seed protein content and soybean yields. CONCLUSIONS: Selection and application of effective soybean rhizobia across China according to biogeography were clarified to promote the SNF, thereby improving soybean yield. SIGNIFICANCE AND IMPACT OF THE STUDY: Rhizobial diversity and biogeography were evaluated systematically in six sites across China. Available iron and soil pH are found to be the most important determinants for the distribution of soybean rhizobia. Inoculation to soybean enhances SNF, positively correlating to the increase in soybean yield and seed protein content.
AIMS: The aim of the study was to survey rhizobial biogeography and to inoculate soybean with selected rhizobia in China to enhance symbiotic nitrogen fixation (SNF). METHODS AND RESULTS: Biogeography, genetic diversity and phylogeny of soybean rhizobia were surveyed. Inocula were prepared and applied to soybean. Results showed that Bradyrhizobium elkanii and Ensifer fredii were widely distributed in acid and alkaline soils respectively. Available iron was detected as the first determinant for distribution of the two rhizobia and the soybean varieties did not greatly affect the rhizobial compatibility. Geographical latitude and precipitation in June were the main geographical and climatic factors affecting the rhizobial distribution. Inoculation with selected rhizobia increased the nodule number, fresh weight, occupation ratio, seed protein content and soybean yields. CONCLUSIONS: Selection and application of effective soybean rhizobia across China according to biogeography were clarified to promote the SNF, thereby improving soybean yield. SIGNIFICANCE AND IMPACT OF THE STUDY: Rhizobial diversity and biogeography were evaluated systematically in six sites across China. Available iron and soil pH are found to be the most important determinants for the distribution of soybean rhizobia. Inoculation to soybean enhances SNF, positively correlating to the increase in soybean yield and seed protein content.
Authors: Francisco Temprano-Vera; Dulce Nombre Rodríguez-Navarro; Sebastian Acosta-Jurado; Xavier Perret; Romain K Fossou; Pilar Navarro-Gómez; Tao Zhen; Deshui Yu; Qi An; Ana Maria Buendía-Clavería; Javier Moreno; Francisco Javier López-Baena; Jose Enrique Ruiz-Sainz; Jose Maria Vinardell Journal: Front Microbiol Date: 2018-11-21 Impact factor: 5.640
Authors: Ricardo A R Machado; Lisa Thönen; Carla C M Arce; Vanitha Theepan; Fausto Prada; Daniel Wüthrich; Christelle A M Robert; Evangelia Vogiatzaki; Yi-Ming Shi; Olivier P Schaeren; Matheus Notter; Rémy Bruggmann; Siegfried Hapfelmeier; Helge B Bode; Matthias Erb Journal: Nat Biotechnol Date: 2020-02-17 Impact factor: 54.908