A C P Motzko-Soares1, R C L Vizin1, T M S Martins2, A R O Hungaro2, J R Sato1,3, M C Almeida1,4, D C Carrettiero1,4. 1. Graduate Program in Neuroscience and Cognition, Universidade Federal do ABC (UFABC), São Bernardo do Campo, SP, Brazil. 2. Undergraduate Program in Science and Technology, Universidade Federal do ABC (UFABC), São Bernardo do Campo, SP, Brazil. 3. Center for Mathematics Computation and Cognition (CMCC), Universidade Federal do ABC (UFABC), São Bernardo do Campo, SP, Brazil. 4. Center for Natural and Human Sciences (CCNH), Universidade Federal do ABC (UFABC), São Bernardo do Campo, SP, Brazil.
Abstract
AIM: Here, we have extensively investigated the relationship between thermoregulation and neurodegeneration-induced dementia of the Alzheimer's type using intracerebroventricular injections of streptozotocin (icv-STZ). METHODS: Male Wistar rats were treated with bilateral injections of icv-STZ, and their thermoregulatory profiles (core body temperature, tail-skin temperature, cold and heat defence responses and thermal place preference) were evaluated. Spatial memory, locomotor activity, social interaction, brain ventricular volume, and Aβ1-42 and tau protein levels in the brain were analysed to characterize the effects of STZ on the brain and behaviour. RESULTS: In addition to deficits in spatial memory, reduced social interaction and an increased brain ventricular volume, icv-STZ rats presented a pattern of hyperthermia, as demonstrated by an increased core body temperature. Hyperthermia was due to the activation of both autonomic heat conservation and behavioural cold avoidance, as STZ-treated rats presented tail-cutaneous vasoconstriction and an altered thermal preference. They also showed a distinct cold defence response when exposed to cold. CONCLUSION: Our data bring evidence that icv-STZ in rats causes hyperthermia, with activation of both autonomic and behavioural thermoregulatory defence responses when challenged at colder temperatures, leading us to hypothesize that they are more efficient in preventing hypothermia. These data are relevant for a better understanding of neurodegenerative disease mechanisms.
AIM: Here, we have extensively investigated the relationship between thermoregulation and neurodegeneration-induced dementia of the Alzheimer's type using intracerebroventricular injections of streptozotocin (icv-STZ). METHODS: Male Wistar rats were treated with bilateral injections of icv-STZ, and their thermoregulatory profiles (core body temperature, tail-skin temperature, cold and heat defence responses and thermal place preference) were evaluated. Spatial memory, locomotor activity, social interaction, brain ventricular volume, and Aβ1-42 and tau protein levels in the brain were analysed to characterize the effects of STZ on the brain and behaviour. RESULTS: In addition to deficits in spatial memory, reduced social interaction and an increased brain ventricular volume, icv-STZrats presented a pattern of hyperthermia, as demonstrated by an increased core body temperature. Hyperthermia was due to the activation of both autonomic heat conservation and behavioural cold avoidance, as STZ-treated rats presented tail-cutaneous vasoconstriction and an altered thermal preference. They also showed a distinct cold defence response when exposed to cold. CONCLUSION: Our data bring evidence that icv-STZ in rats causes hyperthermia, with activation of both autonomic and behavioural thermoregulatory defence responses when challenged at colder temperatures, leading us to hypothesize that they are more efficient in preventing hypothermia. These data are relevant for a better understanding of neurodegenerative disease mechanisms.
Authors: Daniel Moreira-Silva; Robson C L Vizin; Talita M S Martins; Tatiana L Ferreira; Maria C Almeida; Daniel C Carrettiero Journal: Bio Protoc Date: 2019-10-20
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Authors: Daniel Moreira-Silva; Daniel C Carrettiero; Adriele S A Oliveira; Samanta Rodrigues; Joyce Dos Santos-Lopes; Paula M Canas; Rodrigo A Cunha; Maria C Almeida; Tatiana L Ferreira Journal: Front Neurosci Date: 2018-09-21 Impact factor: 4.677