Literature DB >> 2971670

Rapid separation of Pneumocystis carinii from lung lavage fluids.

E L Pesanti1, B A McCarron.   

Abstract

A simple, rapid procedure to separate Pneumocystis carinii obtained by lavage of lungs of steroid-treated rats from rat leukocytes is described. Commercially available monoclonal antibody to rat common leukocyte determinants is used to sediment the leukocytes, resulting in supernatant fluid containing P. carinii cells virtually free of intact rat cells.

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Year:  1988        PMID： 2971670      PMCID： PMC266672          DOI： 10.1128/jcm.26.8.1598-1599.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Pneumocystis carinii: new separation method from lung tissue.

Authors:  P D Walzer; M E Rutledge; K Yoneda; B J Stahr
Journal:  Exp Parasitol       Date:  1979-06       Impact factor: 2.011

2.  Factors responsible for increased susceptibility of mice to intestinal colonization after treatment with streptomycin.

Authors:  J U Que; S W Casey; D J Hentges
Journal:  Infect Immun       Date:  1986-07       Impact factor: 3.441

3.  Improved isolation of rat lung alveolar type II cells. More representative recovery and retention of cell polarity.

Authors:  N K Weller; M J Karnovsky
Journal:  Am J Pathol       Date:  1986-01       Impact factor: 4.307

4.  Uptake and degradation of Pneumocystis carinii by macrophages in vitro.

Authors:  L A Von Behren; E L Pesanti
Journal:  Am Rev Respir Dis       Date:  1978-12

5.  Pneumocystis carinii: oxygen uptake, antioxidant enzymes, and susceptibility to oxygen-mediated damage.

Authors:  E L Pesanti
Journal:  Infect Immun       Date:  1984-04       Impact factor: 3.441

6.  Phospholipid profile of Pneumocystis carinii and its interaction with alveolar type II epithelial cells.

Authors:  E L Pesanti
Journal:  Infect Immun       Date:  1987-03       Impact factor: 3.441

7.  Analysis of Pneumocystis carinii cysts with a fluorescence-activated cell sorter.

Authors:  C R Libertin; G E Woloschak; W R Wilson; T F Smith
Journal:  J Clin Microbiol       Date:  1984-11       Impact factor: 5.948

8.  Isolation of pulmonary alveolar type I cells from adult rats.

Authors:  N K Weller; M J Karnovsky
Journal:  Am J Pathol       Date:  1986-09       Impact factor: 4.307
  8 in total
  1 in total

1.  Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations.

Authors:  J A De Stefano; J M Foy; D W Sullivan; S M Dawes; M T Cushion; G F Babcock; R G Sleight; H van Halbeek; P D Walzer
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289
  1 in total

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