| Literature DB >> 29716370 |
M Holler1, J Raabe1, A Diaz1, M Guizar-Sicairos1, R Wepf2, M Odstrcil1, F R Shaik1, V Panneels1, A Menzel1, B Sarafimov1, S Maag1, X Wang1, V Thominet1, H Walther1, T Lachat3, M Vitins1, O Bunk1.
Abstract
For many scientific questions gaining three-dimensional insight into a specimen can provide valuable information. We here present an instrument called "tOMography Nano crYo (OMNY)," dedicated to high resolution 3D scanning x-ray microscopy at cryogenic conditions via hard X-ray ptychography. Ptychography is a lens-less imaging method requiring accurate sample positioning. In OMNY, this in achieved via dedicated laser interferometry and closed-loop position control reaching sub-10 nm positioning accuracy. Cryogenic sample conditions are maintained via conductive cooling. 90 K can be reached when using liquid nitrogen as coolant, and 10 K is possible with liquid helium. A cryogenic sample-change mechanism permits measurements of cryogenically fixed specimens. We compare images obtained with OMNY with older measurements performed using a nitrogen gas cryo-jet of stained, epoxy-embedded retina tissue and of frozen-hydrated Chlamydomonas cells.Entities:
Mesh：
Year: 2018 PMID： 29716370 DOI： 10.1063/1.5020247
Source DB: PubMed Journal: Rev Sci Instrum ISSN： 0034-6748 Impact factor: 1.523