Torbjörn Ramqvist1, Ramona Gabriela Ursu2, Linnea Haeggblom1, Leila Mirzaie1, Caroline Gahm3, Lalle Hammarstedt-Nordenvall3, Tina Dalianis1, Anders Näsman4. 1. Department of Oncology-Pathology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden. 2. Discipline of Microbiology, Grigore T. Popa University of Medicine and Pharmacy, Iasi, Romania. 3. Department of Clinical Science and Technology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden. 4. Department of Oncology-Pathology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden Anders.Nasman@ki.se.
Abstract
BACKGROUND/AIM: Malignant tumors of the salivary glands are rare and heterogeneous, with more than 20 subtypes, and classified mainly by histopathology. Their diagnosis is often challenging and their etiology unknown. Here, the possible association between human polyomaviruses (PyVs) and one or more salivary gland tumor subtypes was examined. MATERIALS AND METHODS: Ninety-one primary tumors, including 12 subtypes and eight corresponding metastases, were analyzed for the presence of DNA of 10 different human PyV species by a bead-based multiplex assay using polymerase chain reaction and Luminex analyses. RESULTS: Three samples, one adenocarcinoma (not otherwise specified), one adenoid cystic carcinoma, and one mucoepidermoid carcinoma were found to be positive. However, the amount of MCPyV DNA in these tumors was estimated to be less than one genome per tumor cell. CONCLUSION: The analysis of DNA from 10 human PyVs in a large number of malignant salivary gland cancers did not implicate any of these human PyVs as an important causative agent in any of the 12 subtypes studied. Copyright
BACKGROUND/AIM: Malignant tumors of the salivary glands are rare and heterogeneous, with more than 20 subtypes, and classified mainly by histopathology. Their diagnosis is often challenging and their etiology unknown. Here, the possible association between humanpolyomaviruses (PyVs) and one or more salivary gland tumor subtypes was examined. MATERIALS AND METHODS: Ninety-one primary tumors, including 12 subtypes and eight corresponding metastases, were analyzed for the presence of DNA of 10 different human PyV species by a bead-based multiplex assay using polymerase chain reaction and Luminex analyses. RESULTS: Three samples, one adenocarcinoma (not otherwise specified), one adenoid cystic carcinoma, and one mucoepidermoid carcinoma were found to be positive. However, the amount of MCPyV DNA in these tumors was estimated to be less than one genome per tumor cell. CONCLUSION: The analysis of DNA from 10 humanPyVs in a large number of malignant salivary gland cancers did not implicate any of these humanPyVs as an important causative agent in any of the 12 subtypes studied. Copyright
Authors: Mark Zupancic; Stefan Holzhauser; Liquin Cheng; Torbjörn Ramqvist; Juan Du; Signe Friesland; Anders Näsman; Tina Dalianis Journal: Viruses Date: 2022-05-13 Impact factor: 5.818