Luigi Minafra1, Valentina Bravatà2, Francesco P Cammarata1, Giorgio Russo1, Maria C Gilardi1,3, Giusi I Forte1. 1. Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Cefalù, Italy. 2. Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Cefalù, Italy valentina.bravata@ibfm.cnr.it. 3. Nuclear Medicine Unit, San Raffaele Scientific Institute, Milan, Italy.
Abstract
BACKGROUND/AIM: In breast cancer (BC) care, radiation therapy (RT) is an efficient treatment to control localized tumor. Radiobiological research is needed to understand molecular differences that affect radiosensitivity of different tumor subtypes and the response variability. The aim of this study was to analyze gene expression profiling (GEP) in primary BC cells following irradiation with doses of 9 Gy and 23 Gy delivered by intraoperative electron radiation therapy (IOERT) in order to define gene signatures of response to high doses of ionizing radiation. MATERIALS AND METHODS: We performed GEP by cDNA microarrays and evaluated cell survival after IOERT treatment in primary BC cell cultures. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to validate candidate genes. RESULTS: We showed, for the first time, a 4-gene and a 6-gene signature, as new molecular biomarkers, in two primary BC cell cultures after exposure at 9 Gy and 23 Gy respectively, for which we observed a significantly high survival rate. CONCLUSION: Gene signatures activated by different doses of ionizing radiation may predict response to RT and contribute to defining a personalized biological-driven treatment plan. Copyright
BACKGROUND/AIM: In breast cancer (BC) care, radiation therapy (RT) is an efficient treatment to control localized tumor. Radiobiological research is needed to understand molecular differences that affect radiosensitivity of different tumor subtypes and the response variability. The aim of this study was to analyze gene expression profiling (GEP) in primary BC cells following irradiation with doses of 9 Gy and 23 Gy delivered by intraoperative electron radiation therapy (IOERT) in order to define gene signatures of response to high doses of ionizing radiation. MATERIALS AND METHODS: We performed GEP by cDNA microarrays and evaluated cell survival after IOERT treatment in primary BC cell cultures. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to validate candidate genes. RESULTS: We showed, for the first time, a 4-gene and a 6-gene signature, as new molecular biomarkers, in two primary BC cell cultures after exposure at 9 Gy and 23 Gy respectively, for which we observed a significantly high survival rate. CONCLUSION: Gene signatures activated by different doses of ionizing radiation may predict response to RT and contribute to defining a personalized biological-driven treatment plan. Copyright
Authors: Valentina Bravatà; Luigi Minafra; Francesco Paolo Cammarata; Pietro Pisciotta; Debora Lamia; Valentina Marchese; Giada Petringa; Lorenzo Manti; Giuseppe Ap Cirrone; Maria Carla Gilardi; Giacomo Cuttone; Giusi Irma Forte; Giorgio Russo Journal: Br J Radiol Date: 2018-07-05 Impact factor: 3.039
Authors: Francesco P Cammarata; Filippo Torrisi; Giusi I Forte; Luigi Minafra; Valentina Bravatà; Pietro Pisciotta; Gaetano Savoca; Marco Calvaruso; Giada Petringa; Giuseppe A P Cirrone; Anna L Fallacara; Laura Maccari; Maurizio Botta; Silvia Schenone; Rosalba Parenti; Giacomo Cuttone; Giorgio Russo Journal: Int J Mol Sci Date: 2019-09-24 Impact factor: 5.923
Authors: Valentina Bravatà; Walter Tinganelli; Francesco P Cammarata; Luigi Minafra; Marco Calvaruso; Olga Sokol; Giada Petringa; Giuseppe A P Cirrone; Emanuele Scifoni; Giusi I Forte; Giorgio Russo Journal: J Pers Med Date: 2021-04-16