Literature DB >> 29712409

Design of a Molecular Beacon DNA Probe with Two Fluorophores.

Peng Zhang1, Terry Beck2, Weihong Tan1.   

Abstract

Fluorescence resonance energy transfer between two fluorophores (F1 and F2 ) attached to the two ends of a molecular beacon DNA probe containing a hairpin structure can be used for quantitative DNA/RNA studies. Concentrations of target-DNA as low as 1.7×10-10  M could be determined with a commercial spectrometer by using coumarin and 6-carboxyfluorescein as the fluorophores. Measurements on the Förster energy transfer distance for the donor/acceptor pair can also be carried out using these DNA probes.
© 2001 WILEY-VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany.

Entities:  

Keywords:  DNA recognition; donor-acceptor systems; energy transfer; fluorescence spectroscopy

Year:  2001        PMID： 29712409     DOI： 10.1002/1521-3773(20010119)40:2<402::AID-ANIE402>3.0.CO;2-I

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  7 in total

1.  Detection of dihydrofolate reductase conformational change by FRET using two fluorescent amino acids.

Authors:  Shengxi Chen; Nour Eddine Fahmi; Lin Wang; Chandrabali Bhattacharya; Stephen J Benkovic; Sidney M Hecht
Journal:  J Am Chem Soc       Date:  2013-08-22       Impact factor: 15.419

2.  Synthesis, Characterization and Biosensing Application of Photon Upconverting Nanoparticles.

Authors:  Manoj Kumar; Peng Zhang
Journal:  Proc SPIE Int Soc Opt Eng       Date:  2009-02-16

Review 3.  Nucleic-Acid Structures as Intracellular Probes for Live Cells.

Authors:  Devleena Samanta; Sasha B Ebrahimi; Chad A Mirkin
Journal:  Adv Mater       Date:  2019-07-04       Impact factor: 30.849

Review 4.  XNAs: A Troubleshooter for Nucleic Acid Sensing.

Authors:  Tanushree Mana; Budhaditya Bhattacharya; Hiya Lahiri; Rupa Mukhopadhyay
Journal:  ACS Omega       Date:  2022-04-28

5.  Ratiometric electrochemical proximity assay for sensitive one-step protein detection.

Authors:  Kewei Ren; Jie Wu; Feng Yan; Huangxian Ju
Journal:  Sci Rep       Date:  2014-03-12       Impact factor: 4.379

6.  Anodic Electrogenerated Chemiluminescence of Ru(bpy)3(2+) with CdSe Quantum Dots as Coreactant and Its Application in Quantitative Detection of DNA.

Authors:  Yong-Ping Dong; Ting-Ting Gao; Ying Zhou; Li-Ping Jiang; Jun-Jie Zhu
Journal:  Sci Rep       Date:  2015-10-16       Impact factor: 4.379

7.  The detection and identification of dengue virus serotypes with quantum dot and AuNP regulated localized surface plasmon resonance.

Authors:  Ankan Dutta Chowdhury; Kenshin Takemura; Indra Memdi Khorish; Fahmida Nasrin; Mya Myat Ngwe Tun; Kouichi Morita; Enoch Y Park
Journal:  Nanoscale Adv       Date:  2019-12-13
  7 in total

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