| Literature DB >> 29711544 |
Sheldon Yoshio Okada1, Raz Jelinek2, Deborah Charych3.
Abstract
A blue to red color change is induced on addition of phospholipase A2 to modified PDA vesicles 1 (PDA=polydiacetylene). This bathochromic transition results from chemical modification of the vesicles by hydrolysis of the enzyme substrate embedded in the PDA matrix. Addition of a known phospholipase inhibitor or removal of Ca2+ ions suppresses the color change, which suggests the potential for applications in high-throughput screening assays. © 1999 WILEY-VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany.Entities:
Keywords: Biosensors; Conjugation; Enzyme catalysis; Enzyme inhibitors; Vesicles
Year: 1999 PMID： 29711544 DOI： 10.1002/(SICI)1521-3773(19990301)38:5<655::AID-ANIE655>3.0.CO;2-4
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336