Literature DB >> 29707607

Analysis of Mitochondrial Structure in the Body Wall Muscle of Caenorhabditis elegans.

Shaarika Sarasija1, Kenneth R Norman1.   

Abstract

Mitochondrial function is altered in various pathologies, highlighting the crucial role mitochondria plays in maintaining cellular homeostasis. Mitochondrial structure undergoes constant fission and fusion in response to changing cellular environment. Due to this, analyzing mitochondrial structure could provide insight into the physiological state of the cell. In this protocol, we describe a method to analyze mitochondrial structure in body wall muscles in the nematode Caenorhabditis elegans, using both transgenic and dye-based approaches.

Entities:  

Keywords:  C. elegans; Calcium; Mitochondria; Mitochondrial membrane potential; Mitotracker; TMRE

Year:  2018        PMID： 29707607      PMCID： PMC5914529          DOI： 10.21769/BioProtoc.2801

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  7 in total

1.  Secreted VAPB/ALS8 major sperm protein domains modulate mitochondrial localization and morphology via growth cone guidance receptors.

Authors:  Sung Min Han; Hiroshi Tsuda; Youfeng Yang; Jack Vibbert; Pauline Cottee; Se-Jin Lee; Jessica Winek; Claire Haueter; Hugo J Bellen; Michael A Miller
Journal:  Dev Cell       Date:  2012-01-19       Impact factor: 12.270

Review 2.  Maintenance of C. elegans.

Authors:  Theresa Stiernagle
Journal:  WormBook       Date:  2006-02-11

3.  Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.

Authors:  A Fire; S Xu; M K Montgomery; S A Kostas; S E Driver; C C Mello
Journal:  Nature       Date:  1998-02-19       Impact factor: 49.962

4.  Inhibition of tau aggregation in a novel Caenorhabditis elegans model of tauopathy mitigates proteotoxicity.

Authors:  Chronis Fatouros; Ghulam Jeelani Pir; Jacek Biernat; Sandhya Padmanabhan Koushika; Eckhard Mandelkow; Eva-Maria Mandelkow; Enrico Schmidt; Ralf Baumeister
Journal:  Hum Mol Genet       Date:  2012-05-18       Impact factor: 6.150

Review 5.  Calcium, ATP, and ROS: a mitochondrial love-hate triangle.

Authors:  Paul S Brookes; Yisang Yoon; James L Robotham; M W Anders; Shey-Shing Sheu
Journal:  Am J Physiol Cell Physiol       Date:  2004-10       Impact factor: 4.249

6.  Analysis of cell fate from single-cell gene expression profiles in C. elegans.

Authors:  Xiao Liu; Fuhui Long; Hanchuan Peng; Sarah J Aerni; Min Jiang; Adolfo Sánchez-Blanco; John I Murray; Elicia Preston; Barbara Mericle; Serafim Batzoglou; Eugene W Myers; Stuart K Kim
Journal:  Cell       Date:  2009-10-30       Impact factor: 41.582

7.  A γ-Secretase Independent Role for Presenilin in Calcium Homeostasis Impacts Mitochondrial Function and Morphology in Caenorhabditis elegans.

Authors:  Shaarika Sarasija; Kenneth R Norman
Journal:  Genetics       Date:  2015-10-23       Impact factor: 4.562
  7 in total
  3 in total

1.  Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans.

Authors:  Shaarika Sarasija; Kenneth R Norman
Journal:  J Vis Exp       Date:  2019-02-23       Impact factor: 1.355

2.  Presenilin mutations deregulate mitochondrial Ca2+ homeostasis and metabolic activity causing neurodegeneration in Caenorhabditis elegans.

Authors:  Shaarika Sarasija; Jocelyn T Laboy; Zahra Ashkavand; Jennifer Bonner; Yi Tang; Kenneth R Norman
Journal:  Elife       Date:  2018-07-10       Impact factor: 8.140

3.  Modulating the endoplasmic reticulum stress response attenuates neurodegeneration in a Caenorhabditis elegans model of spinal muscular atrophy.

Authors:  James J Doyle; Celine Vrancx; Claudia Maios; Audrey Labarre; Shunmoogum A Patten; J Alex Parker
Journal:  Dis Model Mech       Date:  2020-12-22       Impact factor: 5.758
  3 in total

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