Kishore Ginjupalli1, Tushar Shaw2, Chaitanya Tellapragada3, Ramakrishna Alla4, Lokendra Gupta5, Nagaraja Upadhya Perampalli6. 1. Department of Dental Materials, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India. Electronic address: kishore.gp@manipal.edu. 2. Department of Microbiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India. 3. Manipal Center for Virus Research, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India. 4. Department of Dental Materials, Vishnu Dental College, Bhimavaram, 534202, Andhra Pradesh, India. 5. Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India. 6. Department of Dental Materials, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.
Abstract
OBJECTIVES: The main objective of the present in vitro study is to evaluate the antimicrobial activity and properties of irreversible hydrocolloid impression material incorporated with silver nanoparticles of varying size at different concentrations. METHODS: Silver nanoparticles of 80-100, 50-80, 30-50 and 10-20nm size were added to irreversible hydrocolloid impression material at 0.5, 1.0, 2.0 and 5.0wt%. Antimicrobial activity of the silver nanoparticle incorporated irreversible hydrocolloid was measured using disk diffusion method. The gel strength, flow, gelation time and permanent deformation were measured according to American Dental Association specification #18. Data were analyzed using analysis of variation at a confidence interval of 95% (α=0.05). RESULTS: Silver nanoparticles of 80-100nm size have imparted superior antimicrobial activity to the irreversible hydrocolloid in a dose-dependent manner whereas finer nanoparticle size did not exhibit any antimicrobial activity. The addition of silver nanoparticles did not alter the properties of irreversible hydrocolloid at 0.5 and 1.0wt% whereas at higher concentrations significant differences in flow, gelation time and strength were observed. SIGNIFICANCE: The results of the present study indicate that silver nanoparticles of size range 80-100nm are superior in imparting antimicrobial activity to irreversible hydrocolloid compared to finer particle size range.
OBJECTIVES: The main objective of the present in vitro study is to evaluate the antimicrobial activity and properties of irreversible hydrocolloid impression material incorporated with silver nanoparticles of varying size at different concentrations. METHODS:Silver nanoparticles of 80-100, 50-80, 30-50 and 10-20nm size were added to irreversible hydrocolloid impression material at 0.5, 1.0, 2.0 and 5.0wt%. Antimicrobial activity of the silver nanoparticle incorporated irreversible hydrocolloid was measured using disk diffusion method. The gel strength, flow, gelation time and permanent deformation were measured according to American Dental Association specification #18. Data were analyzed using analysis of variation at a confidence interval of 95% (α=0.05). RESULTS:Silver nanoparticles of 80-100nm size have imparted superior antimicrobial activity to the irreversible hydrocolloid in a dose-dependent manner whereas finer nanoparticle size did not exhibit any antimicrobial activity. The addition of silver nanoparticles did not alter the properties of irreversible hydrocolloid at 0.5 and 1.0wt% whereas at higher concentrations significant differences in flow, gelation time and strength were observed. SIGNIFICANCE: The results of the present study indicate that silver nanoparticles of size range 80-100nm are superior in imparting antimicrobial activity to irreversible hydrocolloid compared to finer particle size range.
Authors: Joanna Wezgowiec; Anna Wieczynska; Mieszko Wieckiewicz; Anna Czarny; Andrzej Malysa; Piotr Seweryn; Marek Zietek; Anna Paradowska-Stolarz Journal: Materials (Basel) Date: 2022-03-31 Impact factor: 3.623