Erik B Finger1, John C Bischof2. 1. Department of Surgery. 2. Departments of Mechanical and Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Abstract
PURPOSE OF REVIEW: The objective of this review is to describe the physical and biological barriers to organ cryopreservation, historic approaches for conventional cryopreservation and evolving techniques for ice-free cryopreservation by vitrification. RECENT FINDINGS: Vitrification is a process whereby a biologic substance is cooled to cryogenic temperatures without the destructive phase transition of liquid to solid ice. Recent advances in cryoprotective solutions, organ perfusion techniques and novel heating technologies have demonstrated the potential for vitrification and rewarming organs on a scale applicable for human transplantation. SUMMARY: Successful strategies for organ cryopreservation could enable organ banking, which would recast the entire process in which organs are recovered, allocated, stored and prepared for transplant.
PURPOSE OF REVIEW: The objective of this review is to describe the physical and biological barriers to organ cryopreservation, historic approaches for conventional cryopreservation and evolving techniques for ice-free cryopreservation by vitrification. RECENT FINDINGS: Vitrification is a process whereby a biologic substance is cooled to cryogenic temperatures without the destructive phase transition of liquid to solid ice. Recent advances in cryoprotective solutions, organ perfusion techniques and novel heating technologies have demonstrated the potential for vitrification and rewarming organs on a scale applicable for human transplantation. SUMMARY: Successful strategies for organ cryopreservation could enable organ banking, which would recast the entire process in which organs are recovered, allocated, stored and prepared for transplant.
Authors: Marlene Davis Ekpo; George Frimpong Boafo; Suleiman Shafiu Gambo; Yuying Hu; Xiangjian Liu; Jingxian Xie; Songwen Tan Journal: Front Vet Sci Date: 2022-06-09
Authors: Reinier J de Vries; Shannon N Tessier; Peony D Banik; Sonal Nagpal; Stephanie E J Cronin; Sinan Ozer; Ehab O A Hafiz; Thomas M van Gulik; Martin L Yarmush; James F Markmann; Mehmet Toner; Heidi Yeh; Korkut Uygun Journal: Nat Protoc Date: 2020-05-20 Impact factor: 13.491