Woan-Ruoh Lee1,2, Shing-Chuan Shen1, Calvin T Sung3, Pei-Ying Liu4, Jia-You Fang5,6,7,8. 1. Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan. 2. Department of Dermatology, Taipei Medical University Shuang Ho Hospital, New Taipei City, Taiwan. 3. School of Medicine, University of California, Riverside, California, USA. 4. Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan, 333, Taiwan. 5. Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan, 333, Taiwan. fajy@mail.cgu.edu.tw. 6. Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan, 333, Taiwan. fajy@mail.cgu.edu.tw. 7. Research Center for Industry of Human Ecology and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Kweishan, Taoyuan, Taiwan. fajy@mail.cgu.edu.tw. 8. Department of Anesthesiology, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan. fajy@mail.cgu.edu.tw.
Abstract
PURPOSE: Most of the investigations into laser-assisted skin permeation have used the intact skin as the permeation barrier. Whether the laser is effective in improving cutaneous delivery via barrier-defective skin is still unclear. METHODS: In this study, ablative (Er:YAG) and non-ablative (Er:glass) lasers were examined for the penetration of peptide and siRNA upon topical application on in vitro skin with a healthy or disrupted barrier. RESULTS: An enhanced peptide flux (6.9 fold) was detected after tape stripping of the pig stratum corneum (SC). A further increase of flux to 11.7 fold was obtained after Er:YAG laser irradiation of the SC-stripped skin. However, the application of Er:glass modality did not further raise the flux via the SC-stripped skin. A similar trend was observed in the case of psoriasiform skin. Conversely, the flux was enhanced 3.7 and 2.6 fold after treatment with the Er:YAG and the Er:glass laser on the atopic dermatitis (AD)-like skin. The 3-D skin structure captured by confocal microscopy proved the distribution of peptide and siRNA through the microchannels and into the surrounding tissue. CONCLUSIONS: The fractional laser was valid for ameliorating macromolecule permeation into barrier-disrupted skin although the enhancement level was lower than that of normal skin.
PURPOSE: Most of the investigations into laser-assisted skin permeation have used the intact skin as the permeation barrier. Whether the laser is effective in improving cutaneous delivery via barrier-defective skin is still unclear. METHODS: In this study, ablative (Er:YAG) and non-ablative (Er:glass) lasers were examined for the penetration of peptide and siRNA upon topical application on in vitro skin with a healthy or disrupted barrier. RESULTS: An enhanced peptide flux (6.9 fold) was detected after tape stripping of the pig stratum corneum (SC). A further increase of flux to 11.7 fold was obtained after Er:YAG laser irradiation of the SC-stripped skin. However, the application of Er:glass modality did not further raise the flux via the SC-stripped skin. A similar trend was observed in the case of psoriasiform skin. Conversely, the flux was enhanced 3.7 and 2.6 fold after treatment with the Er:YAG and the Er:glass laser on the atopic dermatitis (AD)-like skin. The 3-D skin structure captured by confocal microscopy proved the distribution of peptide and siRNA through the microchannels and into the surrounding tissue. CONCLUSIONS: The fractional laser was valid for ameliorating macromolecule permeation into barrier-disrupted skin although the enhancement level was lower than that of normal skin.
Authors: P F Peñas; M Gómez; G F Buezo; L Rios; M Yáñez-Mo; C Cabañas; F Sánchez-Madrid; A García-Díez Journal: J Invest Dermatol Date: 1998-07 Impact factor: 8.551
Authors: Fitsum F Sahle; Tsige Gebre-Mariam; Bodo Dobner; Johannes Wohlrab; Reinhard H H Neubert Journal: Skin Pharmacol Physiol Date: 2014-08-29 Impact factor: 3.479
Authors: Vinzent Kevin Ortner; Nhi Nguyen; Jonathan R Brewer; Vita Solovyeva; Merete Haedersdal; Peter Alshede Philipsen Journal: Lasers Surg Med Date: 2022-04-22