Ahmad Reza Shahverdi1, Faranak Shahverdi2, Elnaz Faghfuri3, Mohammad Reza Khoshayand4, Faranak Mavandadnejad3, Mohammad Hossein Yazdi2, Mohsen Amini5. 1. Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Recombinant Vaccine Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: shahverd@sina.tums.ac.ir. 2. Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. 3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. 4. Department of Food and Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. 5. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUNDS AND AIMS: Selenium nanoparticles (SeNPs) have been reported to exhibit an inhibitory effect on cancer cells. In the present study, we aimed to compare the in vitro and in vivo effects of SeNPs and folic acid surface-coated selenium nanoparticles (FA@SeNPs) on breast cancer. METHODS: FA@SeNPs and SeNPs were chemically synthesized and characterized with different instrumental techniques. The cytotoxicity of both nanomaterials was evaluated against 4T1 cells. In addition, the intravenous administration effect of these nanomaterials (300 μg/week) on the lifespan and tumor size of cancer-bearing mice was investigated. RESULTS: Although the SeNPs showed an antiproliferative effect against the cell line, the cytotoxicity of the FA@SeNPs was higher than that of the SeNPs. A low concentration of FA@SeNPs (25 μg/mL corresponding to 8.75 μg/mL of elemental SeNPs) caused approximately 68% cell mortality. In the in vivo study, the nanomaterials decreased the tumor growth rate in cancerous mice in relation to the control group. FA@SeNPs were more effective than SeNPs. CONCLUSIONS: The combination of SeNPs and FA has a potent antiproliferative effect against 4T1 cells, significantly increases the lifespan, and prevents tumor growth.
BACKGROUNDS AND AIMS: Selenium nanoparticles (SeNPs) have been reported to exhibit an inhibitory effect on cancer cells. In the present study, we aimed to compare the in vitro and in vivo effects of SeNPs and folic acid surface-coated selenium nanoparticles (FA@SeNPs) on breast cancer. METHODS: FA@SeNPs and SeNPs were chemically synthesized and characterized with different instrumental techniques. The cytotoxicity of both nanomaterials was evaluated against 4T1 cells. In addition, the intravenous administration effect of these nanomaterials (300 μg/week) on the lifespan and tumor size of cancer-bearing mice was investigated. RESULTS: Although the SeNPs showed an antiproliferative effect against the cell line, the cytotoxicity of the FA@SeNPs was higher than that of the SeNPs. A low concentration of FA@SeNPs (25 μg/mL corresponding to 8.75 μg/mL of elemental SeNPs) caused approximately 68% cell mortality. In the in vivo study, the nanomaterials decreased the tumor growth rate in cancerousmice in relation to the control group. FA@SeNPs were more effective than SeNPs. CONCLUSIONS: The combination of SeNPs and FA has a potent antiproliferative effect against 4T1 cells, significantly increases the lifespan, and prevents tumor growth.
Authors: Rene Hernandez-Delgadillo; Claudia María García-Cuéllar; Yesennia Sánchez-Pérez; Nayely Pineda-Aguilar; Marco Antonio Martínez-Martínez; Eyra Elvyra Rangel-Padilla; Sergio Eduardo Nakagoshi-Cepeda; Juan Manuel Solís-Soto; Rosa Isela Sánchez-Nájera; María Argelia Akemi Nakagoshi-Cepeda; Shankararaman Chellam; Claudio Cabral-Romero Journal: Int J Nanomedicine Date: 2018-10-05