Afsaneh Golkar-Narenji1,2, Hussein Eimani2,3, Firooz Samadi1, Saeed Hasani1, Abdol-Hossein Shahverdi2, Poopak Eftekhari-Yazdi2, Mohammad Kamalinejad4. 1. Department of Animal Science Gorgan University of Agricultural Sciences and Natural Resources Gorgan Iran. 2. Department of Embryology, Cell Science Research Center, Royan Institute ACECR Tehran Iran. 3. Department of Anatomy, Faculty of Medicine Baqyiatallah (a.s.) University of Medical Sciences Tehran Iran. 4. Department of Pharmacognosy, School of Pharmacy Shaheed Beheshti University of Medical Sciences Tehran Iran.
Abstract
PURPOSE: This experiment examined the effect of Papaver rhoeas L. extract on in vitro maturation, in vitro fertilization (IVF) and subsequent developmental competence of mouse oocytes. MATERIALS AND METHODS: Cumulus oocyte complexes (COCs) at germinal vesicle stage were collected from female Naval Medical Research Institute (NMRI) mouse ovaries. The COCs were transferred to maturation medium supplemented with different concentrations of P. rhoeas extract. Two trials were carried out to examine the effect of low concentrations (0, 10, 15, 20, 25 μg/ml) and high concentrations (0, 50, 100, 200 μg/ml) of the extract. The maturation rate was recorded. After IVF, embryos were cultured and their developmental process was monitored for 96 h. RESULTS: Maturation rate and blastocyst formation improved by using low concentrations of the extract; however, no significant increase was observed when compared to the control group. In addition no significant differences were observed in the fertilization rates of oocytes treated with both low and high concentrations compared to the control group. However, among higher concentrations, 100 μg/ml, P. rhoeas extract significantly increased both the in vitro maturation rate and in vitro developmental (IVD) competence when compared to the control group (P < 0.05). CONCLUSIONS: It was concluded that natural extracts increase the IVD competence of oocytes. The improved effect on oocyte maturation was dependent on the addition of optimum concentrations of P. rhoeas extract to the maturation medium.
PURPOSE: This experiment examined the effect of Papaver rhoeas L. extract on in vitro maturation, in vitro fertilization (IVF) and subsequent developmental competence of mouse oocytes. MATERIALS AND METHODS: Cumulus oocyte complexes (COCs) at germinal vesicle stage were collected from female Naval Medical Research Institute (NMRI) mouse ovaries. The COCs were transferred to maturation medium supplemented with different concentrations of P. rhoeas extract. Two trials were carried out to examine the effect of low concentrations (0, 10, 15, 20, 25 μg/ml) and high concentrations (0, 50, 100, 200 μg/ml) of the extract. The maturation rate was recorded. After IVF, embryos were cultured and their developmental process was monitored for 96 h. RESULTS: Maturation rate and blastocyst formation improved by using low concentrations of the extract; however, no significant increase was observed when compared to the control group. In addition no significant differences were observed in the fertilization rates of oocytes treated with both low and high concentrations compared to the control group. However, among higher concentrations, 100 μg/ml, P. rhoeas extract significantly increased both the in vitro maturation rate and in vitro developmental (IVD) competence when compared to the control group (P < 0.05). CONCLUSIONS: It was concluded that natural extracts increase the IVD competence of oocytes. The improved effect on oocyte maturation was dependent on the addition of optimum concentrations of P. rhoeas extract to the maturation medium.
Entities:
Keywords:
Antioxidant; Embryo; In vitro; Oocyte; Papaver rhoeas L.
Authors: J A Joseph; B Shukitt-Hale; N A Denisova; D Bielinski; A Martin; J J McEwen; P C Bickford Journal: J Neurosci Date: 1999-09-15 Impact factor: 6.167
Authors: Sebastian Schaffer; Gunter P Eckert; Walter E Müller; Rafael Llorach; Diego Rivera; Simona Grande; Claudio Galli; Francesco Visioli Journal: Lipids Date: 2004-12 Impact factor: 1.880
Authors: Mari C W Myhrstad; Harald Carlsen; Olov Nordström; Rune Blomhoff; Jan Øivind Moskaug Journal: Free Radic Biol Med Date: 2002-03-01 Impact factor: 7.376