Herbert Fluhr1, Stefanie Krenzer1, Marek Zygmunt2. 1. Department of Obstetrics and Gynecology, University of Tübingen, Tübingen, and. 2. Department of Obstetrics and Gynecology, University of Greifswald, Greifswald, Germany.
Abstract
Aim: Endometrial tissue inhibitors of metalloproteinases (TIMPs) appear to play an essential role during early implantation by modulating the invasiveness of the trophoblast. The expression of TIMP-1, TIMP-2 and TIMP-3 in human endometrial stromal cells (ESC) was investigated during decidualization in vitro. Methods: Endometrial stromal cells were isolated from hysterectomy specimens from premenopausal women undergoing surgery for benign reasons. Decidualization in vitro was induced by the application of 1 µmol/L progesterone and 30 nmol/L 17β-estradiol over 9 days. The expression of TIMP-1, TIMP-2 and TIMP-3 in ESC was measured by semiquantitative real-time reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay over intervals of 3 days. Results: Decidualization in vitro was confirmed by a significant increase in prolactin expression. TIMP-1 and TIMP-2 mRNA and secreted protein showed no significant changes over the time-course of decidualization. In contrast, TIMP-3 was upregulated during the first 3 days of decidualization. An eightfold upregulation was observed until day 6, and the effect was less pronounced by day 9. Conclusion: These results suggest a regulatory role of the TIMP system for endometrial differentiation in the second half of the menstrual cycle and in early implantation. The expression pattern of endometrial TIMP-3 might be important for the regulation of trophoblast invasion. (Reprod Med Biol 2008; 7: 169-175).
Aim: Endometrial tissue inhibitors of metalloproteinases (TIMPs) appear to play an essential role during early implantation by modulating the invasiveness of the trophoblast. The expression of TIMP-1, TIMP-2 and TIMP-3 in human endometrial stromal cells (ESC) was investigated during decidualization in vitro. Methods: Endometrial stromal cells were isolated from hysterectomy specimens from premenopausal women undergoing surgery for benign reasons. Decidualization in vitro was induced by the application of 1 µmol/L progesterone and 30 nmol/L 17β-estradiol over 9 days. The expression of TIMP-1, TIMP-2 and TIMP-3 in ESC was measured by semiquantitative real-time reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay over intervals of 3 days. Results: Decidualization in vitro was confirmed by a significant increase in prolactin expression. TIMP-1 and TIMP-2 mRNA and secreted protein showed no significant changes over the time-course of decidualization. In contrast, TIMP-3 was upregulated during the first 3 days of decidualization. An eightfold upregulation was observed until day 6, and the effect was less pronounced by day 9. Conclusion: These results suggest a regulatory role of the TIMP system for endometrial differentiation in the second half of the menstrual cycle and in early implantation. The expression pattern of endometrial TIMP-3 might be important for the regulation of trophoblast invasion. (Reprod Med Biol 2008; 7: 169-175).
Entities:
Keywords:
decidualization; endometrium; expression; implantation; tissue inhibitors of metalloproteinases
Authors: T Higuchi; H Kanzaki; H Nakayama; M Fujimoto; H Hatayama; K Kojima; M Iwai; T Mori; J Fujita Journal: Endocrinology Date: 1995-11 Impact factor: 4.736