Masakatsu Fujinoki1, Takeshi Kawamura2, Toshifusa Toda3, Tadashi Ishimoda-Takagi4, Hideki Ohtake1, Nobuyoshi Shimizu2, Makoto Okuno5. 1. Department of Physiology, Dokkyo University School of Medicine, Mibu, Tochigi. 2. Department of Molecular Biology, Keio University School of Medicine, Shinanomachi, Shinjuku-ku, Tokyo. 3. Tokyo Metropolitan Institute of Gerontology Proteomics Collaboration Center, Tokyo Metropolitan Institute of Gerontology, Sakaecho, Itabashi-ku, Tokyo. 4. Department of Biology, Tokyo Gakugei University, Koganei, Tokyo. 5. Department of Life Sciences, Graduate School of Arts and Science, University of Tokyo, Komaba, Meguro-ku, Tokyo, Japan.
Abstract
Background and Aims: Sperm motility is regulated by protein phosphorylation. The 66 kDa protein obtained from hamster sperm flagella was phosphorylated at serine residues associated with the motility initiation. In order to understand the regulatory mechanism of sperm motility, the 66 kDa protein was identified in the present study. Methods: The 66 kDa protein was purified by 2-D gel electrophoresis and identified by matrix-assisted laser desorption ionization mass spectrometry, liquid chromatography-tandem mass spectrometry and peptide sequencer. Results: The 66 kDa protein was tubulin β chain. Conclusion: The 66 kDa protein is one of the tubulin β chain isoforms and phosphorylated in relation to the motility initiation. (Reprod Med Biol 2004; 3: 133-139).
Background and Aims: Sperm motility is regulated by protein phosphorylation. The 66 kDa protein obtained from hamster sperm flagella was phosphorylated at serine residues associated with the motility initiation. In order to understand the regulatory mechanism of sperm motility, the 66 kDa protein was identified in the present study. Methods: The 66 kDa protein was purified by 2-D gel electrophoresis and identified by matrix-assisted laser desorption ionization mass spectrometry, liquid chromatography-tandem mass spectrometry and peptide sequencer. Results: The 66 kDa protein was tubulin β chain. Conclusion: The 66 kDa protein is one of the tubulin β chain isoforms and phosphorylated in relation to the motility initiation. (Reprod Med Biol 2004; 3: 133-139).
Entities:
Keywords:
hamster; liquid chromatography‐tandem mass spectrometry; peptide mass finger printing; phosphorylation; spermatozoa; tubulin