Afsaneh Golkar-Narenji1, Hamid Gourabi1, Hussein Eimani2, Zeinab Barekati3, Aliasghar Akhlaghi4. 1. Department of Genetics Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR No 12, Hafez St, Banihashem St, Resalat Ave 19395-4644 Tehran Iran. 2. Department of Embryology Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR Tehran Iran. 3. Laboratory for Gynecological Oncology, Department of Biomedicine, Women's Hospital University of Basel Basel Switzerland. 4. Department of Epidemiology and Reproductive Health, Reproductive epidemiology Research Center Royan Institute for Reproductive Biomedicine, ACECR Tehran Iran.
Abstract
PURPOSE: To study assisted reproductive technology (ART) protocols including superovulation, in vitro fertilization (IVF) and in vitro development (IVD) for BALB/cJ mice in comparison with a common ART protocol for NMRI mice. METHODS: Adult NMRI and BALB/cJ mice were superovulated using a 48 h G-interval. In order to find a more suitable G-interval for the BALB/cJ strain, G-intervals including 44, 46 and 50 h were also examined. Superovulation rates were recorded in all groups. IVF rate of BALB/c oocytes in T6 and mHTF media were compared. IVD rates of BALB/cJ zygotes in mHTF, T6 and G1V5/G2V5 media were compared. In addition, IVF and IVD rates of BALB/cJ and NMRI oocytes were compared in T6 medium during IVF-IVD procedures. RESULTS: In BALB/cJ mice the highest superovulation rates were observed with 44-46 h G-intervals. However, with a 48 h G-interval, superovulation rates were significantly lower in BALB/cJ compared to NMRI mice (p < 0.05). mHTF medium significantly increased in vitro fertilization of BALB/cJ oocytes compared to T6 medium (p < 0.05). Fertilization rate of NMRI oocytes was significantly higher than BALB/cJ oocytes in T6 medium (p < 0.05). The BALB/cJ embryo IVD was significantly higher in G1/G2 medium compared to mHTF and T6 media (p < 0.01). CONCLUSIONS: Superovulation with 48 h G-interval and using T6 during all in vitro procedures produces embryos more efficiently for NMRI mice than for BALB/cJ mice. For BALB/cJ mice, a protocol including superovulation with a 44-46 h G-interval, using mHTF during IVF and G1V5/G2V5 medium during IVD, may improve in vitro embryo production.
PURPOSE: To study assisted reproductive technology (ART) protocols including superovulation, in vitro fertilization (IVF) and in vitro development (IVD) for BALB/cJ mice in comparison with a common ART protocol for NMRI mice. METHODS: Adult NMRI and BALB/cJ mice were superovulated using a 48 h G-interval. In order to find a more suitable G-interval for the BALB/cJ strain, G-intervals including 44, 46 and 50 h were also examined. Superovulation rates were recorded in all groups. IVF rate of BALB/c oocytes in T6 and mHTF media were compared. IVD rates of BALB/cJ zygotes in mHTF, T6 and G1V5/G2V5 media were compared. In addition, IVF and IVD rates of BALB/cJ and NMRI oocytes were compared in T6 medium during IVF-IVD procedures. RESULTS: In BALB/cJ mice the highest superovulation rates were observed with 44-46 h G-intervals. However, with a 48 h G-interval, superovulation rates were significantly lower in BALB/cJ compared to NMRI mice (p < 0.05). mHTF medium significantly increased in vitro fertilization of BALB/cJ oocytes compared to T6 medium (p < 0.05). Fertilization rate of NMRI oocytes was significantly higher than BALB/cJ oocytes in T6 medium (p < 0.05). The BALB/cJ embryo IVD was significantly higher in G1/G2 medium compared to mHTF and T6 media (p < 0.01). CONCLUSIONS: Superovulation with 48 h G-interval and using T6 during all in vitro procedures produces embryos more efficiently for NMRI mice than for BALB/cJ mice. For BALB/cJ mice, a protocol including superovulation with a 44-46 h G-interval, using mHTF during IVF and G1V5/G2V5 medium during IVD, may improve in vitro embryo production.
Entities:
Keywords:
In vitro development (IVD); In vitro fertilization (IVF); Medium; Mouse strain; Superovulation
Authors: G Sher; V Knutzen; C J Stratton; M M Montakhab; S G Allenson; J Mayville; J A Rubenstein; M J Glass; S M Bilach Journal: Fertil Steril Date: 1984-04 Impact factor: 7.329