Literature DB >> 2969827

Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts.

P E Nielsen1, C Jeppesen, O Buchardt.   

Abstract

Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.

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Year:  1988        PMID： 2969827     DOI： 10.1016/0014-5793(88)81245-6

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  13 in total

1.  Effects of diaminopurine and inosine substitutions on A-tract induced DNA curvature. Importance of the 3'-A-tract junction.

Authors:  N E Mollegaard; C Bailly; M J Waring; P E Nielsen
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

2.  Uranyl photofootprinting of triple helical DNA.

Authors:  P E Nielsen
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

3.  Specific binding of a HeLa cell nuclear protein to RNA sequences in the human immunodeficiency virus transactivating region.

Authors:  R Gaynor; E Soultanakis; M Kuwabara; J Garcia; D S Sigman
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

4.  Uranyl mediated photofootprinting reveals strong E. coli RNA polymerase--DNA backbone contacts in the +10 region of the DeoP1 promoter open complex.

Authors:  C Jeppesen; P E Nielsen
Journal:  Nucleic Acids Res       Date:  1989-07-11       Impact factor: 16.971

5.  High resolution footprinting of EcoRI and distamycin with Rh(phi)2(bpy)3+, a new photofootprinting reagent.

Authors:  K Uchida; A M Pyle; T Morii; J K Barton
Journal:  Nucleic Acids Res       Date:  1989-12-25       Impact factor: 16.971

6.  Sequence-dependent reactivity of linear DNA to chemical cleavage by Cu(II):thiol combinations including cysteine or glutathione.

Authors:  D C John; K T Douglas
Journal:  Biochem J       Date:  1993-01-15       Impact factor: 3.857

Review 7.  From cisplatin to artificial nucleases--the role of metal ion-nucleic acid interactions in biology.

Authors:  B Lippert
Journal:  Biometals       Date:  1992       Impact factor: 2.949

8.  DNA conformational analysis in solution by uranyl mediated photocleavage.

Authors:  P E Nielsen; N E Møllegaard; C Jeppesen
Journal:  Nucleic Acids Res       Date:  1990-07-11       Impact factor: 16.971

9.  The influence of the 2-amino group of guanine on DNA conformation. Uranyl and DNase I probing of inosine/diaminopurine substituted DNA.

Authors:  C Bailly; N E Møllegaard; P E Nielsen; M J Waring
Journal:  EMBO J       Date:  1995-05-01       Impact factor: 11.598

10.  Uranyl photoprobing of a four-way DNA junction: evidence for specific metal ion binding.

Authors:  N E Møllegaard; A I Murchie; D M Lilley; P E Nielsen
Journal:  EMBO J       Date:  1994-04-01       Impact factor: 11.598
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