Literature DB >> 2969415

Complement C3d,g/Epstein-Barr virus receptor density on human B-lymphocytes estimated by immunoenzymatic assay and immunocytochemistry.

R Rask1, J M Rasmussen, H V Hansen, P Bysted, S E Svehag.   

Abstract

The density of the C3d,g/Epstein-Barr virus receptor (CR2) on human mononuclear cells (MNC) isolated from CPD stabilized blood, was determined by comparing the concentration of CR2 antigen in extracts from Raji cells, with a known receptor density, with extracts of MNC. The concentration of CR2 was determined by a sandwich ELISA using two mutually non-inhibiting monoclonal anti CR2 antibodies (HB5 and OKB7). The percentage of CR2+ MNC was determined by an immunocytochemical staining method. Analysis of MNC from 20 healthy donors, revealed that the density of CR2 molecules varied from 3,600-7,700 on CR2+ cells, and that the percentage of CR2+ MNC had a mean of 13%.

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Year:  1988        PMID： 2969415

Source DB:  PubMed          Journal:  J Clin Lab Immunol        ISSN： 0141-2760


  3 in total

1.  Biorecognition of HPMA copolymer-adriamycin conjugates by lymphocytes mediated by synthetic receptor binding epitopes.

Authors:  V Omelyanenko; P Kopecková; R K Prakash; C D Ebert; J Kopecek
Journal:  Pharm Res       Date:  1999-07       Impact factor: 4.200

2.  Binding and cytotoxicity of HPMA copolymer conjugates to lymphocytes mediated by receptor-binding epitopes.

Authors:  Aijun Tang; Pavla Kopeiková; Jindrich Kopeckevá
Journal:  Pharm Res       Date:  2003-03       Impact factor: 4.200

3.  Two-step fluorescence screening of CD21-binding peptides with one-bead one-compound library and investigation of binding properties of N-(2-hydroxypropyl)methacrylamide copolymer-peptide conjugates.

Authors:  Hui Ding; Wolfgang M Prodinger; Jindrich Kopecek
Journal:  Biomacromolecules       Date:  2006-11       Impact factor: 6.988
  3 in total

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