Mark Fishbein1, Shannon C K Straub2, Julien Boutte2, Kimberly Hansen3, Richard C Cronn4, Aaron Liston3. 1. Department of Plant Biology, Ecology & Evolution, Oklahoma State University, Stillwater, OK, 74078, USA. 2. Department of Biology, Hobart and William Smith Colleges, Geneva, NY, 14456, USA. 3. Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331, USA. 4. Pacific Northwest Research Station, USDA Forest Service, Corvallis, OR, 97331, USA.
Abstract
PREMISE OF THE STUDY: Leaf surface traits, such as trichome density and wax production, mediate important ecological processes such as anti-herbivory defense and water-use efficiency. We present a phylogenetic analysis of Asclepias plastomes as a framework for analyzing the evolution of trichome density and presence of epicuticular waxes. METHODS: We produced a maximum-likelihood phylogeny using plastomes of 103 species of Asclepias. We reconstructed ancestral states and used model comparisons in a likelihood framework to analyze character evolution across Asclepias. KEY RESULTS: We resolved the backbone of Asclepias, placing the Sonoran Desert clade and Incarnatae clade as successive sisters to the remaining species. We present novel findings about leaf surface evolution of Asclepias-the ancestor is reconstructed as waxless and sparsely hairy, a macroevolutionary optimal trichome density is supported, and the rate of evolution of trichome density has accelerated. CONCLUSIONS: Increased sampling and selection of best-fitting models of evolution provide more resolved and robust estimates of phylogeny and character evolution than obtained in previous studies. Evolutionary inferences are more sensitive to character coding than model selection.
PREMISE OF THE STUDY: Leaf surface traits, such as trichome density and wax production, mediate important ecological processes such as anti-herbivory defense and water-use efficiency. We present a phylogenetic analysis of Asclepias plastomes as a framework for analyzing the evolution of trichome density and presence of epicuticular waxes. METHODS: We produced a maximum-likelihood phylogeny using plastomes of 103 species of Asclepias. We reconstructed ancestral states and used model comparisons in a likelihood framework to analyze character evolution across Asclepias. KEY RESULTS: We resolved the backbone of Asclepias, placing the Sonoran Desert clade and Incarnatae clade as successive sisters to the remaining species. We present novel findings about leaf surface evolution of Asclepias-the ancestor is reconstructed as waxless and sparsely hairy, a macroevolutionary optimal trichome density is supported, and the rate of evolution of trichome density has accelerated. CONCLUSIONS: Increased sampling and selection of best-fitting models of evolution provide more resolved and robust estimates of phylogeny and character evolution than obtained in previous studies. Evolutionary inferences are more sensitive to character coding than model selection.
Keywords:
Apocynaceae; ancestral state reconstruction; data partitioning; evolutionary trend; leaf epidermis; milkweed; model of character evolution; plastid genome; stochastic character mapping; water-use efficiency
Authors: Kevin Weitemier; Shannon C K Straub; Mark Fishbein; C Donovan Bailey; Richard C Cronn; Aaron Liston Journal: PeerJ Date: 2019-09-20 Impact factor: 2.984