Soon Hee Kim1, Yeung Kyu Yeon1, Jung Min Lee1, Janet Ren Chao2, Young Jin Lee1, Ye Been Seo1, Md Tipu Sultan1, Ok Joo Lee1, Ji Seung Lee1, Sung-Il Yoon3, In-Sun Hong4, Gilson Khang5, Sang Jin Lee6, James J Yoo6, Chan Hum Park7,8. 1. Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 24252, Republic of Korea. 2. School of Medicine, George Washington University, Washington, D.C., 20037, USA. 3. Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon, 24341, Republic of Korea. 4. Department of Molecular Medicine, School of Medicine, Gachon University, Incheon, 406-840, Republic of Korea. 5. Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Chonbuk National University, Jeonju, 54896, Republic of Korea. 6. Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157, USA. 7. Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 24252, Republic of Korea. hlpch@paran.com. 8. Departments of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, Chuncheon, 24252, Republic of Korea. hlpch@paran.com.
Abstract
Although three-dimensional (3D) bioprinting technology has gained much attention in the field of tissue engineering, there are still several significant engineering challenges to overcome, including lack of bioink with biocompatibility and printability. Here, we show a bioink created from silk fibroin (SF) for digital light processing (DLP) 3D bioprinting in tissue engineering applications. The SF-based bioink (Sil-MA) was produced by a methacrylation process using glycidyl methacrylate (GMA) during the fabrication of SF solution. The mechanical and rheological properties of Sil-MA hydrogel proved to be outstanding in experimental testing and can be modulated by varying the Sil-MA contents. This Sil-MA bioink allowed us to build highly complex organ structures, including the heart, vessel, brain, trachea and ear with excellent structural stability and reliable biocompatibility. Sil-MA bioink is well-suited for use in DLP printing process and could be applied to tissue and organ engineering depending on the specific biological requirements.
Although three-dimensional (3D) bioprinting technology has gained much attention in the field of tissue engineering, tn class="Chemical">here are still several significant engineering challenges to overcome, including lack of bioink with biocompatibility and printability. Here, we show a bioink created from silk fibroin (SF) for digital light processing (DLP) 3D bioprinting in tissue engineering applications. The SF-based bioink (Sil-MA) was produced by a methacrylation process using glycidyl methacrylate (GMA) during the fabrication of SF solution. The mechanical and rheological properties of Sil-MA hydrogel proved to be outstanding in experimental testing and can be modulated by varying theSil-MA contents. This Sil-MA bioink allowed us to build highly complex organ structures, including theheart, vessel, brain, trachea and ear with excellent structural stability and reliable biocompatibility. Sil-MA bioink is well-suited for use in DLP printing process and could be applied to tissue and organ engineering depending on the specific biological requirements.
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