Denglu Zhang1,2, Kailin Li3,2, Chao Sun3,2, Guangshang Cao1, Yuanfu Qi1, Zhaomin Lin3, Yanxia Guo3, Zhiyong Liu1, Yuan Chen3,2, Jiaxin Liu1, Guanghui Cheng3,2, Peng Wang3,2, Lu Zhang3, Jianye Zhang4, Jiliang Wen4, Dawei Xu5,6, Feng Kong3,2,6, Shengtian Zhao1,4,2,6. 1. The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, The Second Hospital of Shandong University, Jinan, China. 2. Key Laboratory for Kidney Regeneration of Shandong Province, Jinan, China. 3. Department of Central Research Laboratory, The Second Hospital of Shandong University, Jinan, China. 4. Department of Urology, The Second Hospital of Shandong University, Jinan, China. 5. Department of Medicine, Division of Hematology and Centre for Molecular Medicine, Karolinska University Hospital Solna and Karolinska Institutet, Stockholm, Sweden. 6. Shandong University-Karolinska Institutet Collaborative Laboratory for Cancer Research, Jinan, China.
Abstract
OBJECTIVE: To evaluate the potential anti-prostate cancer effects of Paris polyphylla ethanol extract (PPEE) and its underlying mechanisms. MATERIALS AND METHODS: The anti-proliferation activity of PPEE was tested on PC3 and DU145 cells using Cell Counting Kit-8 assay. The pro-apoptotic and cell cycle arrest effects of PPEE were confirmed by flow cytometry. Apoptosis of prostate cancer cells was induced by PPEE through endogenous and exogenous pathways. A mouse xenograft model was used to examine its anti-prostate cancer effects in vivo. RESULTS: We found that the IC50 of PPEE on PC3 cells was 3.98 µg/ml and the IC50 of PPEE on DU145 cells was 8 µg/ml. PPEE induced prostate cancer cell apoptosis in a concentration dependent manner, through endogenous and exogenous pathways. PPEE induced PC3 cell cycle arrest in G0/G1 and G2/M phases, while in DU145cell it induced cell arrest in the G0/G1 phase. PPEE inhibited the growth of prostate cancer cells in vivo. CONCLUSION: PPEE could inhibit prostate cancer growth in vitro and in vivo, induce apoptosis of prostate cancer cells, and cause cell cycle arrest, which laid the foundation for further research on the anti-tumor mechanism of PPEE.
OBJECTIVE: To evaluate the potential anti-prostate cancer effects of Paris polyphylla ethanol extract (PPEE) and its underlying mechanisms. MATERIALS AND METHODS: The anti-proliferation activity of PPEE was tested on PC3 and DU145 cells using Cell Counting Kit-8 assay. The pro-apoptotic and cell cycle arrest effects of PPEE were confirmed by flow cytometry. Apoptosis of prostate cancer cells was induced by PPEE through endogenous and exogenous pathways. A mouse xenograft model was used to examine its anti-prostate cancer effects in vivo. RESULTS: We found that the IC50 of PPEE on PC3 cells was 3.98 µg/ml and the IC50 of PPEE on DU145 cells was 8 µg/ml. PPEE induced prostate cancer cell apoptosis in a concentration dependent manner, through endogenous and exogenous pathways. PPEE induced PC3 cell cycle arrest in G0/G1 and G2/M phases, while in DU145cell it induced cell arrest in the G0/G1 phase. PPEE inhibited the growth of prostate cancer cells in vivo. CONCLUSION: PPEE could inhibit prostate cancer growth in vitro and in vivo, induce apoptosis of prostate cancer cells, and cause cell cycle arrest, which laid the foundation for further research on the anti-tumor mechanism of PPEE.
Entities:
Keywords:
Cell appotosis; Cycle arrest; Paris polyphylla; Prostate cancer
Authors: M E O'Callaghan; E Raymond; J Campbell; A D Vincent; K Beckmann; D Roder; S Evans; J McNeil; J Millar; J Zalcberg; M Borg; K Moretti Journal: Prostate Cancer Prostatic Dis Date: 2017-06-06 Impact factor: 5.554