| Literature DB >> 29692423 |
Matthew T Dinday1, Kelly M Girskis1, Sunyoung Lee2, Scott C Baraban1, Robert F Hunt3.
Abstract
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Year: 2018 PMID: 29692423 PMCID: PMC5915572 DOI: 10.1038/s41598-018-24610-w
Source DB: PubMed Journal: Sci Rep ISSN: 2045-2322 Impact factor: 4.379
Figure 1Fewer PV+ and SST+, but not CR+, GABAergic interneurons in Pafah1b1+/− dentate gyrus. (a) Immunostaining for GAD67-GFP (green), PV (magenta) and DAPI (blue) in wild-type and Pafah1b1+/− mice at P30. (b) Immunostaining for GAD67-GFP (green), SST (magenta) and DAPI (blue) in wild-type and Pafah1b1+/− mice at P30. (c) Immunostaining for GAD67-GFP (green), CR (magenta) and DAPI (blue) in wild-type and Pafah1b1+/− mice at P30. (d–f) Quantification of PV- (d), SST- (e) and CR-expressing (f) GABA neurons in dentate gyrus of wild-type and Pafah1b1+/− mice at P30 (n = 4 for each genotype). Error bars represent s.e.m., *p < 0.05, **p < 0.01.
Figure 2Dramatic change in synaptic inhibition and excitation in dentate granule cells of Pafah1b1+/− mice. (a) Example voltage-clamp recordings of sIPSCs and sEPSCs recorded from granule cells in slices of a wild-type control and Pafah1b1 mutant. (b–d) Average sEPSC frequency (b), sIPSC frequency (c) and sEPSC:sIPSC ratio (d) in granule cells of wild-type and Pafah1b1+/− mice. (e–g) Average mEPSC frequency (e), mIPSC frequency (f) and mEPSC:sIPSC ratio (g) in granule cells of wild-type and Pafah1b1+/− mice. Error bars represent s.e.m., *p < 0.05, **p < 0.01.