Literature DB >> 29687388

Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates.

Jie Li1,2, J Lowry Curley1,2, Z Elizabeth Floyd3, Xiying Wu1,2, Yuan Di C Halvorsen4, Jeffrey M Gimble5,6.   

Abstract

Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion.

Entities:  

Keywords:  Adipose-derived stem cells (ASCs); Biopsy; Collagenase; Expansion; Human; Isolation; Lipoaspirate; Mesenchymal stem cells (MSCs); Stromal vascular fraction (SVF)

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Year:  2018        PMID： 29687388     DOI： 10.1007/978-1-4939-7799-4_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Safety of Human Adipose Stromal Vascular Fraction Cells Isolated with a Closed System Device in an Immunocompetent Murine Pressure Ulcer Model.

Authors:  Joanna Bukowska; Andrea Alarcon Uquillas; Xiying Wu; Trivia Frazier; Katarzyna Walendzik; Mikaela Vanek; Dina Gaupp; Bruce A Bunnell; Paul Kosnik; Babak Mehrara; Adam J Katz; Barbara Gawronska-Kozak; Jeffrey M Gimble
Journal:  Stem Cells Dev       Date:  2020-02-24       Impact factor: 3.272

2.  Adipose-derived stromal/stem cells are verified to be potential seed candidates for bio-root regeneration in three-dimensional culture.

Authors:  Yu Yuan; Xiaonan Zhang; Yuzhen Zhan; Song Tang; Pingmeng Deng; Zhenxiang Wang; Jie Li
Journal:  Stem Cell Res Ther       Date:  2022-06-03       Impact factor: 8.079

3.  Tumor protein p53-induced nuclear protein 2 modulates osteogenic differentiation of human adipose derived stem/stromal cells by activating Wnt/β-catenin signaling.

Authors:  Shi Dong; Jie Li; Xiaonan Zhang
Journal:  Am J Transl Res       Date:  2020-10-15       Impact factor: 4.060

4.  Safety and Efficacy of Human Adipose-Derived Stromal/Stem Cell Therapy in an Immunocompetent Murine Pressure Ulcer Model.

Authors:  Joanna Bukowska; Andrea Alarcon Uquillas; Xiying Wu; Trivia Frazier; Katarzyna Walendzik; Mikaela Vanek; Dina Gaupp; Bruce A Bunnell; Paul Kosnik; Babak Mehrara; Adam J Katz; Barbara Gawronska-Kozak; Jeffrey M Gimble
Journal:  Stem Cells Dev       Date:  2020-02-12       Impact factor: 3.272

5.  MLR-1023 Treatment in Mice and Humans Induces a Thermogenic Program, and Menthol Potentiates the Effect.

Authors:  Candida J Rebello; Ann A Coulter; Andrew G Reaume; Weina Cong; Luke A Cusimano; Frank L Greenway
Journal:  Pharmaceuticals (Basel)       Date:  2021-11-22

6.  Impact of low-intensity pulsed ultrasound on transcription and metabolite compositions in proliferation and functionalization of human adipose-derived mesenchymal stromal cells.

Authors:  Denggao Huang; Yuanhui Gao; Shunlan Wang; Wei Zhang; Hui Cao; Linlin Zheng; Yang Chen; Shufang Zhang; Jie Chen
Journal:  Sci Rep       Date:  2020-08-13       Impact factor: 4.379
  6 in total

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