Literature DB >> 29687386

Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis.

Gail Kilroy1, Marilyn Dietrich1, Xiying Wu2, Jeffrey M Gimble2, Z Elizabeth Floyd3.   

Abstract

Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Adipose tissue stromal vascular cells or primary adipocytes derived from murine adipose tissue and grown in culture are essential tools for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stromal/stem cells along with protocols for inducing adipogenesis in this cell population or isolating the adipose stromal vascular fraction cells for flow cytometric analysis.

Entities:  

Keywords:  Adipogenesis; Adipose-derived stem cells (ASCs); Collagenase; Flow cytometry; Hematopoietic stem cells (HSCs); Isolation; Mesenchymal stem cells (MSCs); Murine; Stromal vascular fraction (SVF)

Mesh：

Substances：

Year:  2018        PMID： 29687386      PMCID： PMC6391981          DOI： 10.1007/978-1-4939-7799-4_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Deletion of LDLRAP1 Induces Atherosclerotic Plaque Formation, Insulin Resistance, and Dysregulated Insulin Response in Adipose Tissue.

Authors:  Tani Leigh; Tatsuo Kawai; Kyle Preston; Sheri Kelemen; Rachael Okune; Amanda St Paul; Cali Corbett; Amanda M Peluzzo; Jun Yu; Rosario G Scalia; Michael V Autieri
Journal:  Am J Pathol       Date:  2022-04-20       Impact factor: 5.770

Review 2.  Weighing the Risk: effects of Obesity on the Mammary Gland and Breast Cancer Risk.

Authors:  Lauren E Hillers-Ziemer; Lisa M Arendt
Journal:  J Mammary Gland Biol Neoplasia       Date:  2020-06-09       Impact factor: 2.673

3.  Extracellular vesicles from anoxia preconditioned mesenchymal stem cells alleviate myocardial ischemia/reperfusion injury.

Authors:  Chengyu Mao; Dongjiu Li; En Zhou; Erhe Gao; Tiantian Zhang; Shufang Sun; Lin Gao; Yuqi Fan; Changqian Wang
Journal:  Aging (Albany NY)       Date:  2021-02-12       Impact factor: 5.682

4.  Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism.

Authors:  Jacqueline Taylor; Julia Sellin; Lars Kuerschner; Lennart Krähl; Yasmin Majlesain; Irmgard Förster; Christoph Thiele; Heike Weighardt; Elvira Weber
Journal:  Sci Rep       Date:  2020-12-03       Impact factor: 4.379

5.  Mitochondria Transfer from Adipose Stem Cells Improves the Developmental Potential of Cryopreserved Oocytes.

Authors:  Udayanga Sanath Kankanam Gamage; Shu Hashimoto; Yuki Miyamoto; Tatsuya Nakano; Masaya Yamanaka; Akiko Koike; Manabu Satoh; Yoshiharu Morimoto
Journal:  Biomolecules       Date:  2022-07-21

6.  Upregulated integrin α11 in the stroma of cutaneous squamous cell carcinoma promotes skin carcinogenesis.

Authors:  Guillermo A Martínez-Nieto; Hanna-Riikka Teppo; Noora Petrelius; Valerio Izzi; Raman Devarajan; Tiina Petäistö; Hengshuo Liu; Kris S Kim; Sanna-Maria Karppinen; Heli Ruotsalainen; Jarkko Koivunen; Joni M Mäki; Gilbert C Walker; Taina Pihlajaniemi; Donald Gullberg; Ritva Heljasvaara
Journal:  Front Oncol       Date:  2022-08-08       Impact factor: 5.738

7.  A STAT5-Smad3 dyad regulates adipogenic plasticity of visceral adipose mesenchymal stromal cells during chronic inflammation.

Authors:  Jayeeta Giri; Pradyut K Paul; Rahul Das; Nicole Froelich; Raghavan Chinnadurai; Sara McCoy; Wade Bushman; Jacques Galipeau
Journal:  NPJ Regen Med       Date:  2022-08-31

Review 8.  Modeling Obesity-Driven Pancreatic Carcinogenesis-A Review of Current In Vivo and In Vitro Models of Obesity and Pancreatic Carcinogenesis.

Authors:  Sally Kfoury; Patrick Michl; Laura Roth
Journal:  Cells       Date:  2022-10-10       Impact factor: 7.666

9.  Adaptive thermogenesis enhances the life-threatening response to heat in mice with an Ryr1 mutation.

Authors:  Hui J Wang; Chang Seok Lee; Rachel Sue Zhen Yee; Linda Groom; Inbar Friedman; Lyle Babcock; Dimitra K Georgiou; Jin Hong; Amy D Hanna; Joseph Recio; Jong Min Choi; Ting Chang; Nadia H Agha; Jonathan Romero; Poonam Sarkar; Nicol Voermans; M Waleed Gaber; Sung Yun Jung; Matthew L Baker; Robia G Pautler; Robert T Dirksen; Sheila Riazi; Susan L Hamilton
Journal:  Nat Commun       Date:  2020-10-09       Impact factor: 17.694

10.  Exploring the Cell Stemness and the Complexity of the Adipose Tissue Niche.

Authors:  Nadav Kislev; Roza Izgilov; Raizel Adler; Dafna Benayahu
Journal:  Biomolecules       Date:  2021-12-19
  10 in total

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