| Literature DB >> 29687379 |
Anne-Laure Hafner1, Tala Mohsen-Kanson1,2, Christian Dani3.
Abstract
In this chapter, we describe a protocol to induce at a high rate the differentiation of brown/brown like adipocyte progenitors (BAPs) derived from human induced pluripotent stem cells (hiPSCs). We also describe culture conditions to maintain hiPSCs and to derive hiPSC-BAPs.This novel culture system provides an unlimited source of human brown adipocytes and a unique means for studying events regulating the generation and recruitment of human BAPs.Entities:
Keywords: Adipocyte; Adipocyte progenitors; Brown adipocytes; Differentiation; Human induced pluripotent stem cells
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Year: 2018 PMID: 29687379 DOI: 10.1007/978-1-4939-7799-4_4
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745