Literature DB >> 29685054

Analysis of antigen conservation and inactivation of gamma-irradiated avian influenza virus subtype H9N2.

Bahareh Salehi1, Farahnaz Motamedi-Sedeh2, Omid Madadgar1, Iraj Khalili3, Arash Ghalyan Chi Langroudi1, Hermann Unger4, Viskam Wijewardana4.   

Abstract

Avian influenza (AI) A subtype H9N2 virus belongs to Orthomyxoviridae family and causes low-pathogenic disease AI. The use of gamma-irradiated viral antigens has been developed in the production of effective vaccines. In this research, LPAIV H9N2 strain, A/Chicken/IRN/Ghazvin/2001, was multiplied on SPF eggs and irradiated by a Nordian gamma cell instrument. Irradiated and non-irradiated AI virus (AIV) samples were titrated by EID50 method and hemagglutinin (HA) antigen was analyzed by HA test as the WHO pattern method. Infectivity of irradiated virus was determined by egg inoculation method during four blind cultures. The results showed that after increasing the dose of gamma radiation, virus titer gradually decreased. D10 value and optimum dose for complete virus inactivation were calculated by dose/response curve, 3.36 and 29.52 kGy, respectively. In addition, HA antigenicity of gamma-irradiated virus samples from 0 to 30 kGy was not changed. The results of safety test for gamma-irradiated AIV samples showed complete inactivation with gamma ray doses 30 and 35 kGy, without any multiplication on eggs after four blind cultures. According to the results of HA antigen assay and safety test, the gamma-irradiated and complete inactivated AIV subtype H9N2 is a good candidate as an inactivated immunogenic agent for poultry vaccination.

Entities:  

Keywords:  avian influenza A subtype H9N2 virus; gamma irradiation; hemagglutinin antigen; inactivation

Mesh：

Substances：

Year:  2018        PMID： 29685054     DOI： 10.1556/030.65.2018.025

Source DB:  PubMed          Journal:  Acta Microbiol Immunol Hung        ISSN： 1217-8950            Impact factor:   2.048


  5 in total

1.  Inactivated or damaged? Comparing the effect of inactivation methods on influenza virions to optimize vaccine production.

Authors:  José Herrera-Rodriguez; Aurora Signorazzi; Marijke Holtrop; Jacqueline de Vries-Idema; Anke Huckriede
Journal:  Vaccine       Date:  2019-02-11       Impact factor: 3.641

Review 2.  Advances in Irradiated Livestock Vaccine Research and Production Addressing the Unmet Needs for Farmers and Veterinary Services in FAO/IAEA Member States.

Authors:  Hermann Unger; Richard T Kangethe; Fatima Liaqat; Gerrit J Viljoen
Journal:  Front Immunol       Date:  2022-03-28       Impact factor: 7.561

3.  Vaccination With a Gamma Irradiation-Inactivated African Swine Fever Virus Is Safe But Does Not Protect Against a Challenge.

Authors:  Jutta Pikalo; Luca Porfiri; Valerij Akimkin; Hanna Roszyk; Katrin Pannhorst; Richard Thiga Kangethe; Viskam Wijewardana; Julia Sehl-Ewert; Martin Beer; Giovanni Cattoli; Sandra Blome
Journal:  Front Immunol       Date:  2022-04-26       Impact factor: 8.786

4.  Improved Whole Gamma Irradiated Avian Influenza Subtype H9N2 Virus Vaccine Using Trehalose and Optimization of Vaccination Regime on Broiler Chicken.

Authors:  Farahnaz Motamedi Sedeh; Iraj Khalili; Viskam Wijewardana; Hermann Unger; Parvin Shawrang; Mehdi Behgar; Sayed Morteza Moosavi; Arash Arbabi; Sayedeh Maede Hosseini
Journal:  Front Vet Sci       Date:  2022-07-12

5.  Carboxymethyl chitosan bounded iron oxide nanoparticles and gamma-irradiated avian influenza subtype H9N2 vaccine to development of immunity on mouse and chicken.

Authors:  Farahnaz Motamedi-Sedeh; Atefeh Saboorizadeh; Iraj Khalili; Massomeh Sharbatdaran; Viskam Wijewardana; Arash Arbabi
Journal:  Vet Med Sci       Date:  2021-12-08
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.