Yingchuan Li1,2, Xiaoqing Pan1, Michelle L Roberts1, Pengyuan Liu1,3, Theodore A Kotchen4, Allen W Cowley1, David L Mattson1, Yong Liu1, Mingyu Liang1, Srividya Kidambi4. 1. Center of Systems Molecular Medicine, Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA. 2. Department of Critical Care Medicine, Shanghai JiaoTong University affiliated The Sixth People's Hospital, Shanghai, China. 3. Department of Respiratory Medicine, Sir Run Run Shaw Hospital & Institute of Translational Medicine, Zhejiang University, Zhejiang, China. 4. Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA.
Abstract
AIM: To test whether DNA samples stored for a prolonged period (20 years) under various storage conditions could be used for comparative methylation studies using reduced representation bisulfite sequencing. PATIENTS & METHODS: Five groups of human blood DNA samples (n = 5-6/group) were compared. The groupings were based on the anticoagulant used and storage temperature and duration. RESULTS: Methylation profiles of defined genomic regions in the DNA or blood samples archived for 20 years were similar across all storage temperatures, including 4°C. The level of intersample similarity in archived samples was not significantly different than that in recently collected samples. CONCLUSION: Archived samples, including DNA stored at 4°C for 20 years, are suitable for comparative studies of DNA methylation.
AIM: To test whether DNA samples stored for a prolonged period (20 years) under various storage conditions could be used for comparative methylation studies using reduced representation bisulfite sequencing. PATIENTS & METHODS: Five groups of human blood DNA samples (n = 5-6/group) were compared. The groupings were based on the anticoagulant used and storage temperature and duration. RESULTS: Methylation profiles of defined genomic regions in the DNA or blood samples archived for 20 years were similar across all storage temperatures, including 4°C. The level of intersample similarity in archived samples was not significantly different than that in recently collected samples. CONCLUSION: Archived samples, including DNA stored at 4°C for 20 years, are suitable for comparative studies of DNA methylation.
Authors: Michelle L Roberts; Theodore A Kotchen; Xiaoqing Pan; Yingchuan Li; Chun Yang; Pengyuan Liu; Tao Wang; Purushottam W Laud; Thomas H Chelius; Yannick Munyura; David L Mattson; Yong Liu; Allen W Cowley; Srividya Kidambi; Mingyu Liang Journal: Hypertension Date: 2022-01-07 Impact factor: 10.190
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