| Literature DB >> 29680048 |
Fábio Junior Moreira Novaes1, Ivaldo Itabaiana Junior2, Felipe Korbus Sutili3, Philip John Marriott4, Humberto Ribeiro Bizzo5, Francisco Radler de Aquino Neto6, Rodrigo Octávio Mendonça Alves de Souza7, Claudia Moraes Rezende8.
Abstract
Cafestol and kahweol (C&K), two coffee diterpene alcohols with structural similarity which exhibit anticarcinogenic effects, were isolated from green coffee Arabica beans, followed by their lipase-catalysed esterification and purification by preparative high-performance liquid chromatography (HPLC). The isolation and enzymatic synthesis parameters of C&K esters were studied, with the latter optimised by a Central Composite Design; both procedures were monitored by gas chromatography. Scale up and improved isolation conditions resulted in 1.29 g of C&K, with 98% purity from 300 g of green Arabica beans. The highest C&K ester yields were observed using an alcohol:fatty acid molar ratio of 1:5, 73.3 mg mL-1 of CAL-B enzyme, 70 °C and 240 rpm for 3 days in toluene, leading to 85-88% conversion among a variety of tested C&K esters, including n-C14:0-C20:0, C18:1, C18:2 and C18:3.Entities:
Keywords: Cafestol; Cafestol ester; Coffee diterpenes; Ester synthesis; Kahweol; Kahweol ester; Lipase catalyst; Preparative scale isolation
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Year: 2018 PMID： 29680048 DOI： 10.1016/j.foodchem.2018.03.111
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514