Literature DB >> 29675784

Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research.

BarboraBarbora Dvořánková1, Lukáš Lacina2, Karel Smetana1.   

Abstract

Cancer-associated fibroblasts (CAFs) represent a crucial component of cancer microenvironment. CAFs significantly influence biological properties of various types of cancer in terms of local aggressiveness, recurrence, and metastatic behaviour. This chapter summarizes a simple protocol for isolation of normal fibroblasts and their cancer-associated counterparts from normal human skin and mucosa, respectively, as well as from samples of human tumours such as basal/squamous carcinoma, melanoma, and breast cancer, and employment of this procedure for other types of cancer is possible. Isolated fibroblasts can be expanded in vitro and employed for further analysis of, e.g., DNA, RNA, protein, etc.

Entities:  

Keywords:  Cancer microenvironment; Cancer-associated fibroblast; Cell cultivation; Fibroblast; Fibroblast isolation

Mesh：

Year:  2019        PMID： 29675784     DOI： 10.1007/7651_2018_137

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Desmoplastic Crosstalk in Pancreatic Ductal Adenocarcinoma Is Reflected by Different Responses of Panc-1, MIAPaCa-2, PaTu-8902, and CAPAN-2 Cell Lines to Cancer-associated/Normal Fibroblasts.

Authors:  Štepán Novák; Michal Kolář; Arpád Szabó; Zdena Vernerová; Lukáš Lacina; Hynek Strnad; Jana Šáchová; Miluše Hradilová; Jan Havránek; Michal Španko; Matúš Čoma; Lukáš Urban; Miriam Kaňuchová; Nikola Melegová; Robert Gürlich; Josef Dvořák; Karel Smetana; Peter Gál; Pavol Szabo
Journal:  Cancer Genomics Proteomics       Date:  2021 May-Jun       Impact factor: 4.069

2.  Genistein does not inhibit TGF-beta1-induced conversion of human dermal fibroblasts to myofibroblasts.

Authors:  M Kaňuchová; L Urban; N Melegová; M Čoma; B Dvořánková; Karel Smetana; P Gál
Journal:  Physiol Res       Date:  2021-09-10       Impact factor: 1.881

3.  Single-Cell RNA Sequencing Unravels Heterogeneity of the Stromal Niche in Cutaneous Melanoma Heterogeneous Spheroids.

Authors:  Jiří Novotný; Karolína Strnadová; Barbora Dvořánková; Šárka Kocourková; Radek Jakša; Pavel Dundr; Václav Pačes; Karel Smetana; Michal Kolář; Lukáš Lacina
Journal:  Cancers (Basel)       Date:  2020-11-10       Impact factor: 6.639

Review 4.  Tumor-Associated Macrophages and Inflammatory Microenvironment in Gastric Cancer: Novel Translational Implications.

Authors:  Karim Rihawi; Angela Dalia Ricci; Alessandro Rizzo; Stefano Brocchi; Giovanni Marasco; Luigi Vincenzo Pastore; Fabiola Lorena Rojas Llimpe; Rita Golfieri; Matteo Renzulli
Journal:  Int J Mol Sci       Date:  2021-04-07       Impact factor: 5.923

5.  Exosomes produced by melanoma cells significantly influence the biological properties of normal and cancer-associated fibroblasts.

Authors:  Karolína Strnadová; Lucie Pfeiferová; Petr Přikryl; Barbora Dvořánková; Erik Vlčák; Jana Frýdlová; Martin Vokurka; Jiří Novotný; Jana Šáchová; Miluše Hradilová; Jan Brábek; Jana Šmigová; Daniel Rösel; Karel Smetana; Michal Kolář; Lukáš Lacina
Journal:  Histochem Cell Biol       Date:  2021-11-27       Impact factor: 4.304

6.  Cancer associated fibroblasts secreted exosomal miR-1290 contributes to prostate cancer cell growth and metastasis via targeting GSK3β.

Authors:  Shuo Wang; Yudong Cao; Jinchao Ma; Peng Du; Xiao Yang; Ziyi Yu; Yong Yang
Journal:  Cell Death Discov       Date:  2022-08-23

7.  Serum proteomic analysis of melanoma patients with immunohistochemical profiling of primary melanomas and cultured cells: Pilot study.

Authors:  Jan Kučera; Karolína Strnadová; Barbora Dvořánková; Lukáš Lacina; Ivana Krajsová; Jiří Štork; Hana Kovářová; Helena Kupcová Skalníková; Petr Vodička; Jan Motlík; Pavel Dundr; Karel Smetana; Ondřej Kodet
Journal:  Oncol Rep       Date:  2019-09-17       Impact factor: 3.906
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.